Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic

Authors
Hamšíkova, Zuzana; Silaghi, Cornelia; Rudolf, Ivo; Venclíková, Kristýna; Mahríkova, Lenka; Slovák, Mirko; Mendel, Jan; Blažejová, Hana; Berthová, Lenka; Kocianová, Elena; Hubálek, Zdeněk; Schnittger, Leonhard; Kazimírová, Mária
Publication Year
2016
Language
English
Format
article
Status
Versión aceptada para publicación
Description
By amplification and sequencing of 18S rRNA gene fragments, Hepatozoon spp. DNA was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing Ixodes ricinus ticks from Slovakia and Czech Republic, respectively. Hepatozoon spp. DNA was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from Slovakia. Prevalence of infection was significantly higher in Myodes glareolus (11.45 %) than in Apodemus spp. (0.28 %) (P < 0.001). Sequencing of 18S rRNA Hepatozoon spp. gene amplicons from I. ricinus showed 100 % identity with Hepatozoon canis isolates from red foxes or dogs in Europe. Phylogenetic analysis showed that at least two H. canis 18S rRNA genotypes exist in Slovakia of which one was identified also in the Czech Republic. The finding of H. canis in questing I. ricinus suggests the geographical spread of the parasite and a potential role of other ticks as its vectors in areas where Rhipicephalus sanguineus is not endemic. Sequencing of 18S rRNA gene amplicons from M. glareolus revealed the presence of two closely related genetic variants, Hepatozoon sp. SK1 and Hepatozoon sp. SK2, showing 99–100 % identity with isolates from M. glareolus from other European countries. Phylogenetic analysis demonstrates that 18S rRNA variants SK1 and SK2 correspond to previously described genotypes UR1 and UR2 of H. erhardovae, respectively. The isolate from Apodemus flavicollis (Hepatozoon sp. SK3b) was 99 % identical with isolates from reptiles in Africa and Asia. Further studies are necessary to identify the taxonomic status of Hepatozoon spp. parasitizing rodents in Europe and the host-parasite interactions in natural foci.
Fil: Hamšíková, Zuzana. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Silaghi, Cornelia. Ludwig-Maximilians-Universität. Comparative Tropical Medicine and Parasitology; Alemania. University of Zurich. Institute of Parasitology. National Centre for Vector Entomology; Suiza
Fil: Rudolf, Ivo. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa
Fil: Venclíková, Kristýna. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa. The Czech Academy of Sciences. Institute of Macromolecular Chemistry; República Checa
Fil: Mahrikova, Lenka. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Slovak, Mirko. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Mendel, Jan. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa.
Fil: Blažejová, Hana. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa.
Fil: Berthova, Lenka. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia
Fil: Kocianova, Elena. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia
Fil: Hubálek, Zdeněk. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa
Fil: Schnittger, Leonhard. INTA. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kazimirová, Mária. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Source
Parasitology research 115 (10) : 3897–3904. (October 2016)
Subject
Ixodes Ricinus
Metastigmata
Hepatozoon
Sporozoa
Roedores
Rodents
Garrapatas
Apicomplexa
Eslovaquia
República Checa
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/1347