An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle

Authors
Campero, Lucía María; Moreno-Gonzalo, Javier; Venturini, María C.; Moré, Gastón; Dellarupe, Andrea; Rambeaud, Magdalena; Echaide, Ignacio Eduardo; Valentini, Beatriz Susana; Campero, Carlos Manuel; Moore, Prando Dadin; Cano, Dora Beatriz; Fort, Marcelo Cristian; Mota, Rinaldo A.; Serrano-Martínez, Marcos E.; Cruz-Vázquez, Carlos; Ortega-Mora, Luis M.; Álvarez-García, Gema
Publication Year
2018
Language
English
Format
article
Status
Published version
Description
We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1–7) and three enzyme-linked immunosorbent assays (ELISAs 1–3; two in-house and one commercial). Diagnostic performance of the serological tests was calculated and compared according to the following criteria: (1) the “Pre-test information,” which uses previous epidemiological and serological data; (2) the “Majority of tests,” which classifies a serum as positive or negative according to the results obtained by most tests evaluated. Unexpectedly, six tests showed either sensitivity (Se) or specificity (Sp) values lower than 90%. In contrast, the best tests in terms of Se, Sp, and area under the ROC curve (AUC) values were IFAT 1 and optimized ELISA 1 and ELISA 2. We evaluated a high number of IFATs, which are the most widely used tests in Ibero-America. The significant discordances observed among the tests regardless of the criteria employed hinder control programs and urge the use of a common test or with similar performances to either the optimized IFAT 1 and ELISAs 1 and 2.
EEA Rafaela
Fil: Campero, Lucía María. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Moreno-Gonzalo, Javier. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España
Fil: Venturini, María C. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina
Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Dellarupe, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rambeaud, Magdalena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina
Fil: Echaide, Ignacio Eduardo. INTA. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Valentini, Beatriz Susana. INTA. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Campero, Carlos Manuel. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cano, Dora Beatriz. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Fort, Marcelo Cristian. INTA. Estación Experimental Agropecuaria Anguil; Argentina
Fil: Mota, Rinaldo A. Rural Federal University of Pernambuco. Veterinary Medicine Department; Brasil
Fil: Serrano-Martínez, Marcos E. Universidad Peruana Cayetano Heredia. Facultad de Medicina Veterinaria y Zootecnica; Perú
Fil: Cruz-Vázquez, Carlos. Instituto Técnico El Llano; México
Fil: Ortega-Mora, Luis M. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España
Fil: Álvarez-García, Gema. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España
Source
Tropical Animal Health and Production 50 (1) : 75–84 (January 2018)
Subject
Ganado Bovino
Neospora caninum
Anticuerpos
Experimentación en Laboratorio
Técnicas Inmunológicas
Cattle
Antibodies
Laboratory Experimentation
Immunological Techniques
Ibero América
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/2908