Identification and characterization of a new potyvirus infecting cucurbits

Authors
Perotto, Maria Cecilia; Pozzi, E.A.; Celli, Marcos Giovani; Luciani, Cecilia E.; Mitidieri, Mariel Silvina; Conci, Vilma Cecilia
Publication Year
2018
Language
English
Format
article
Status
Published version
Description
A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.
Instituto de Patología Vegetal
Fil: Perotto, Maria Cecilia. INTA. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pozzi, E.A. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina
Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina
Fil: Luciani, Cecilia E. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina
Fil: Mitidieri, Mariel Silvina. INTA. Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Conci, Vilma Cecilia. INTA. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Source
Archives of virology 163 (3) : 719–724. (March 2018)
Subject
Potyviruses
Identification
Plant Viruses
Squashes
Potyvirus
Cucurbitaceae
Identificación
Virus de las Plantas
Calabazas
San Pedro, Buenos Aires
Illumina Sequencing
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/3632