Prevalence and viability of group A rotavirus in dairy farm water sources

Authors
Castells, M.L.; Schild, Carlos Omar; Caffarena, Rubén Dario; Bok, Marina; Giannitti, Federico; Armendano, Joaquín Ignacio; Riet‐Correa, Franklin; Victoria, M.; Parreño, Viviana; Colina, Rodney
Publication Year
2018
Language
English
Format
article
Status
Published version
Description
Aim: To analyse group A rotavirus (RVA) environmental contamination in waters used for calves' consumption and to assess viral viability in dairy farm water sources. Methods and Results: We analysed 202 samples of water used for calves' consumption and RVA was detected by RT‐qPCR in 35·1% (95% CI: 28·9–42·0%). A marked pattern of seasonality was observed with higher frequency of detection in colder than warmer months (P = 0·002). There was no association between viral load and season or between the number of milking cows in the herd and the detection of RVA in the farm. The viability of the RVA particles detected was confirmed by isolation of RVA in cell culture from 5 of 10 water samples. Furthermore, an RVA waterborne outbreak of neonatal calf diarrhoea was described. Conclusions: We demonstrate that RVA is frequent in dairy farm waters, and that the virus is infectious and capable of generating a diarrhoea outbreak. Significance and Impact of the Study: Neonatal diarrhoea syndrome leads to economic losses to the livestock industry worldwide. To determine transmission routes is essential to take action in this regard and reduce the impact that this syndrome has for the livestock production. The results obtained in this work alert the dairy industry and highlight that mitigation strategies are crucial to improve the microbiological quality of this water.
Instituto de Virología
Fil: Castells, M.L. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay
Fil: Schild, Carlos Omar. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay
Fil: Caffarena, Rubén Dario. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Giannitti, Federico. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay. University of Minnesota. College of Veterinary Medicine. Veterinary Population Medicine Department; Estados Unidos
Fil: Armendano, Joaquín Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Riet‐Correa, Franklin. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay
Fil: Victoria, M. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Colina, Rodney. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay
Source
Journal of applied microbiology 124 (3) : 922-929. (March 2018)
Subject
Rotavirus
Granjas Lecheras
Enfermedades de los Animales
Morbosidad
Agua
Dairy Farms
Animal Diseases
Morbidity
Water
Rotavirus A
Prevalencia de una enfermedad
Fuentes de Agua
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/2230