A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle

Authors
Verdes, José Manuel; Márquez, Mercedes; Calliari, Aldo; Battes, Daniel; Moraña, José Antonio; Gimeno, Eduardo Juan; Odriozola, Ernesto Raul; Giannitti, Federico; Guerrero, Florentina; Fidalgo, Luis Eusebio; Pumarola, Martí
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, β-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti–glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver–Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death.
EEA Balcarce
Fil: Verdes, José Manuel. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay
Fil: Márquez, Mercedes. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; España
Fil: Calliari, Aldo. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay
Fil: Battes, Daniel. Universidad de la República. Facultad de Veterinaria; Uruguay.
Fil: Moraña, José Antonio. Universidad de la República. Facultad de Veterinaria; Uruguay.
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias; Argentina
Fil: Odriozola, Ernesto Raul. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Giannitti, Federico. Instituto Nacional de Investigaciones Agropecuarias. La Estanzuela, Colonia; Uruguay
Fil: Guerrero, Florentina. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departments of Anatomy and Animal Production; España
Fil: Fidalgo, Luis Eusebio. Universidad de Santiago de Compostela. Facultad de Veterinaria. Veterinary Clinical Sciences; España
Fil: Pumarola, Martí. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; España
Source
Journal of Veterinary Diagnostic Investigation 27 (3) : 278-286 (May 2015)
Subject
Ganado Bovino
Enfermedades de los Animales
Envenenamiento
Solanum
Cattle
Animal Diseases
Poisoning
Intoxicación
Solanum bonariense
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/4834