Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes

Authors
Salloum, María Soraya; Menduni, Maria Florencia; Benavides, María Patricia; Larrauri, Mariana; Luna, Celina Mercedes; Silvente, Sonia
Publication Year
2018
Language
English
Format
article
Status
Published version
Description
Modern breeding programs might have caused a reduction in plant responsiveness to arbuscular mycorrhizal fungi (AMF). Flavonoids and polyamines (PAs) are hypothesized to play a role in this symbiosis. We tested the effects of them in AMF roots of improved (I-1) and unimproved (UI-4) soybean genotypes, under the hypothesis that domestication decreased their concentration in roots, affecting AMF colonization, particularly arbuscule formation. After 20 days of treatment, AMF roots of UI-4 genotype had greater amount of total flavonoids/phenols and PAs while in I-1 genotype no differences were observed between roots of mycorrhizal (M) and non mycorrhizal (NM) plants. Exogenous application of flavonoids led to an increase in arbuscules in both genotypes. Improved-1 genotype needed higher levels of flavonoids to reach the percentage of mycorrhization achieved by UI-4 control. In regard to PAs, mycorrhizal plants of both genotypes had higher endogenous concentration than NM plants, however, the highest concentration, especially of putrescine (put) was in UI-4 M genotype. To check the participation of put in symbiosis we used RNAi silencing methodology. Down regulation of the GmADC transcript, involved in put formation, had a profound negative effect on mycorrhizal colonization and also affected the normal development of the plant. By contrast, down regulation of GmDAO, in which ADC transcript was expressed, arbuscule formation was similar to control plant. Our results suggest that mycorrhizal colonization is affected by soybean domestication particularly arbuscule formation and this effect seems to be mediated by the endogenous roots levels of flavonoids and PAs, especially put.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Salloum, María Soraya. INTA. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Menduni, Maria Florencia. INTA. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Fondo para la Investigación Científica y Tecnológica; Argentina
Fil: Benavides, María Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Larrauri, Mariana. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Luna, Celina Mercedes. INTA. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silvente, Sonia. Universidad Nacional de Chilecito. Instituto de Ambiente de Montaña y Regiones Áridas (IAMRA); Argentina
Source
Symbiosis 76 (3) : 265–275. (November 2018)
Subject
Soja
Glycine Max
Poliaminas
Flavonoides
Genotipos
Micorrizas Arbusculares
Soybeans
Polyamines
Flavonoids
Genotypes
Arbuscular Mycorrhiza
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/3675