Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants

Authors
Perez Aguirreburialde, Maria Sol; Gomez, Maria Cristina; Ostachuck, Agustín; Wolman, Federico Javier; Albanesi, Guillermo Luis; Pecora, Andrea; Odeon, Anselmo Carlos; Ardila, Fernando Jorge; Escribano, José M.; Dus Santos, Maria Jose; Wigdorovitz, Andres
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Bovine viral diarrhea virus (BVDV) is considered an important cause of economic loss within bovine herds worldwide. In Argentina, only the use of inactivated vaccines is allowed, however, the efficacy of inactivated BVDV vaccines is variable due to its low immunogenicity. The use of recombinant subunit vaccines has been proposed as an alternative to overcome this difficulty. Different studies on protection against BVDV infection have focused the E2 protein, supporting its putative use in subunit vaccines. Utilization of transgenic plants expressing recombinant antigens for the formulation of experimental vaccines represents an innovative and cost effective alternative to the classical fermentation systems. The aim of this work was to develop transgenic alfalfa plants (Medicago sativa, L.) expressing a truncated version of the structural protein E2 from BVDV fused to a molecule named APCH, that target to antigen presenting cells (APCH-tE2). The concentration of recombinant APCH-tE2 in alfalfa leaves was 1 μg/g at fresh weight and its expression remained stable after vegetative propagation. A methodology based an aqueous two phases system was standardized for concentration and partial purification of APCH-tE2 from alfalfa. Guinea pigs parentally immunized with leaf extracts developed high titers of neutralizing antibodies. In bovine, the APCH-tE2 subunit vaccine was able to induce BVDV-specific neutralizing antibodies. After challenge, bovines inoculated with 3 μg of APCH-tE2 produced in alfalfa transgenic plants showed complete virological protection.
Instituto de Virología
Fil: Pérez Aguirreburualde, María Sol. INTA. Instituto de Virología; Argentina
Fil: Gomez, Maria Cristina. INTA. Instituto de Genética; Argentina
Fil: Ostachuck, Agustín. INTA. Instituto de Virología; Argentina
Fil: Wolman, Federico Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina.
Fil: Albanesi, Guillermo Luis. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Pecora, Andrea. INTA. Instituto de Virología; Argentina
Fil: Odeon, Anselmo Carlos. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Ardila, Fernando Jorge. INTA. Instituto de Genética; Argentina
Fil: Escribano, José M. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departamento de Biotecnología; España
Fil: Dus Santos, Maria Jose. INTA. Instituto de Virología; Argentina
Fil: Wigdorovitz, Andrés. INTA. Instituto de Virología; Argentina
Source
Veterinary Immunology and Immunopathology 151 (3–4) : 315-324. (February 2013)
Subject
Ganado Bovino
Enfermedades de los Animales
Vacuna
Diarrea Viral Bovina
Medicago Sativa
Plantas Transgénicas
Cattle
Animal Diseases
Vaccines
Bovine Viral Diarrhoea
Transgenic Plants
Alfalfa
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/4317