Construction of low-ethanol-wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene

Authors
Cuello, Raúl Andrés; Flores Montero, Karina Johana; Mercado, Laura Analia; Combina, Mariana; Ciklic, Ivan Francisco
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
We propose an alternative GMO based strategy to obtain Saccharomyces cerevisiae mutant strains with a slight reduction in their ability to produce ethanol, but with a moderate impact on the yeast metabolism. Through homologous recombination, two truncated Pdc2p proteins Pdc2pΔ344 and Pdc2p Δ519 were obtained and transformed into haploid and diploid lab yeast strains. In the pdc2Δ344 mutants the DNA-binding and transactivation site of the protein remain intact, whereas in pdc2Δ519only the DNA-binding site is conserved. Compared to the control, the diploid BY4743 pdc2Δ519 mutant strain reduced up to 7.4% the total ethanol content in lab scale-vinifications. The residual sugar and volatile acidity was not significantly affected by this ethanol reduction. Remarkably, we got a much higher ethanol reduction of 10 and 15% when the pdc2Δ519 mutation was tested in a native and a commercial wine yeast strain against their respective controls. Our results demonstrate that the insertion of the pdc2Δ519 mutation in wine yeast strains can reduce the ethanol concentration up to 1.89% (v/v) without affecting the fermentation performance. In contrast to non-GMO based strategies, our approach permits the insertion of the pdc2Δ519 mutation in any locally selected wine strain, making possible to produce quality wines with regional characteristics and lower alcohol content. Thus, we consider our work a valuable contribution to the problem of high ethanol concentration in wine
EEA Mendoza
Fil: Cuello, Raúl Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. INTA. Estación Experimental Agropecuaria Mendoza; Argentina.
Fil: Flores Montero, Karina Johana. INTA. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Mercado, Laura Analia. INTA. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. INTA. Estación Experimental Agropecuaria Mendoza; Argentina.
Fil: Ciklic, Ivan Francisco. INTA. Estación Experimental Agropecuaria Mendoza; Argentina
Source
AMB Express 7 : 67 (2017)
Subject
Saccharomyces Cerevisiae
Levadura
Etanol
Ingeniería Genética
Vinos
Yeasts
Ethanol
Genetic Engineering
Wines
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/1410