A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies

Authors
Marrero Diaz De Villegas, Rubén; Rodríguez Limardo, Ramiro; Carrillo, Elisa Cristina; Konig, Guido Alberto; Turjanski, Adrián G.
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
Foot and mouth disease is caused by a non-enveloped virus (FMDV), which disposes several antigenic sites at the surface of their capsid proteins. The most relevant and immunodominant antigenic site of FMDV (site A or AnSA) includes a key virus–cell interaction element (RGD motif) located in the Viral Protein 1 (VP1), more precisely at the GH loop. AnSA includes a set of overlapped and mainly linear epitopes, which are the main targets of the humoral immune response. Taking advantage over specific structural features of the GH loop, we have evaluated the influence of every amino acid residue at AnSA in the interaction with 2 neutralizing antibodies by molecular modeling techniques. Additionally, we constructed diverse interaction complexes with multiple site A mutants and discussed about the structural influence of amino acidic insertions in such relevant antigenic site of FMDV. Our approach is in agreement with previous ELISA experiments and allows the understanding of how FMDV mutations may alter the interaction with different antibodies, as we can estimate the contribution of each amino acid to the interaction. Overall, our work contributes to the development of specific vaccination strategies for FMD control.
Instituto de Biotecnología
Fil: Marrero Diaz De Villegas, Rubén. INTA. Instituto de Biotecnología; Argentina
Fil: Rodríguez Limardo, Ramiro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Bioinformática Estructural; Argentina
Fil: Carrillo, Elisa Cristina. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Konig, Guido Alberto. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Turjanski, Adrian G. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Source
Journal of Immunological Methods 425 : 51-57 (October 2015)
Subject
Fiebre Aftosa
Enfermedades de los Animales
Virus Fiebre Aftosa
Anticuerpos Monoclonales
Bioinformática
Foot and Mouth Disease
Animal Diseases
Aphthovirus
Monoclonal Antibodies
Bioinformatics
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/5301