Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice

Authors
Lavari, Luisina; Burns, Patricia Graciela; Paez, Roxana Beatriz; Reinheimer, Jorge Alberto; Vinderola, Celso Gabriel
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
Aims: To evaluate the effects of spray drying of Lactobacillus rhamnosus 64 on its capacity to modulate the gut immune response and on the attenuation of TNBS‐induced colitis in mice. Methods and Results: Lactobacillus rhamnosus 64 was spray dried in cheese whey‐starch solution and administered to mice for 3, 6 or 10 consecutive days. Peritoneal macrophage phagocytic activity, secretory IgA levels in the small intestinal fluid and TNFα, IFNγ, IL‐10, IL‐6 and IL‐2 levels in homogenates of the small and large intestine were determined. The effects of spray drying were also evaluated in an acute model of Trinitrobenzenesulfonic acid (TNBS)‐induced colitis. A shift in the regulation of immune parameters, particularly the cytokine profile, was observed for mice treated with the spray‐dried culture, compared to the profile observed in animals that received the strain as fresh culture (FC). The spray‐dried culture of L. rhamnosus 64 showed anti‐inflammatory properties in murine model of TNBS‐induced colitis. Conclusions: The spray‐drying process of L. rhamnosus 64 in whey‐starch modified its immunomodulating capacity in healthy animals and conferred enhanced protection in an in vivo model of inflammation.
EEA Rafaela
Fil: Lavari, Luisina. INTA. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Fil: Paez, Roxana Beatriz. INTA. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Source
Journal of applied microbiology 123 (4) : 992-1002. (October 2017)
Subject
Lactobacillus
Probióticos
Lactosuero
Almidón
Ratón
Probiotics
Whey
Starch
Mice
Spray Drying
Secado por Pulverización
Lactobacillus rhamnosus
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/3291