Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter

Authors
Decima Oneto, Cecilia Andrea; Otegui, Marí­a Elena; Baroli, Irene; Beznec, Ailin; Faccio, Paula Daniela; Bossio, Adrian Ezequiel; Blumwald, Eduardo; Lewi, Dalia Marcela
Publication Year
2016
Language
English
Format
article
Status
Versión aceptada para publicación
Description
Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity and improved tolerance to abiotic stress. The aim of this study was to generate andcharacterize transgenic plants of maize (Zea mays L.) transformed with the IPT gene sequence underthe regulation of SARK promoter (protein kinase receptor-associated senescence). Three independenttransgenic events and their segregating null controls were evaluated in two watering regimes (WW:well watered; WD: water deficit) imposed for two weeks around anthesis. Our results show that the WD treatment induced IPT expression with the concomitant increase in cytokinin levels, which prolongedthe persistence of total green leaf area, and maintained normal photosynthetic rate and stomatal con-ductance. These trends were accompanied by a minor decrease in number of grains per plant, individualgrain weight and plant grain yield as compared to WW plants. Plants expressing the IPT gene under WD had PGR, anthesis and silking dates and biomass levels similar to WW plants. Our results demonstratethat expression of the IPT gene under the regulation of the SARK promoter helps improve productivity under WD conditions in C4 plants like maize.
Inst. de Genética "Ewald A. Favret"- IGEAF
Fil: Decima Oneto, Cecilia Andrea. INTA. Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Otegui, Marí­a Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Baroli, Irene. INTA. Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beznec, Ailin. INTA. Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Faccio, Paula Daniela. INTA. Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Bossio, Adrian EzequielINTA. Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Blumwald, Eduardo. University of California. Department Plant Sciences; Estados Unidos
Fil: Lewi, Dalia Marcela. INTA. Instituto de Genética "Ewald A. Favret"; Argentina
Source
Journal of biotechnology 220 : 66-77. (February 2016)
Subject
Genética
Agua
Estrés de Sequia
Maíz
Resistencia a la Sequía
Genetics
Water
Drought Stress
Maize
Drought Resistance
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/1124