Association mapping in sunflower for sclerotinia head rot resistance

Authors
Fusari, Corina Mariana; Di Rienzo, Julio A.; Troglia, Carolina Beatriz; Nishinakamasu, Veronica; Moreno, Maria Valeria; Maringolo, Carla Andrea; Quiroz, Facundo Jose; Alvarez, Daniel; Escande, Alberto; Hopp, Horacio Esteban; Heinz, Ruth Amelia; Lia, Veronica Viviana; Paniego, Norma Beatriz
Publication Year
2012
Language
English
Format
article
Status
Published version
Description
Background: Sclerotinia Head Rot (SHR) is one of the most damaging diseases of sunflower in Europe, Argentina, and USA, causing average yield reductions of 10 to 20 %, but leading to total production loss under favorable environmental conditions for the pathogen. Association Mapping (AM) is a promising choice for Quantitative Trait Locus (QTL) mapping, as it detects relationships between phenotypic variation and gene polymorphisms in existing germplasm without development of mapping populations. This article reports the identification of QTL for resistance to SHR based on candidate gene AM. Results: A collection of 94 sunflower inbred lines were tested for SHR under field conditions using assisted inoculation with the fungal pathogen Sclerotinia sclerotiorum. Given that no biological mechanisms or biochemical pathways have been clearly identified for SHR, 43 candidate genes were selected based on previous transcript profiling studies in sunflower and Brassica napus infected with S. sclerotiorum. Associations among SHR incidence and haplotype polymorphisms in 16 candidate genes were tested using Mixed Linear Models (MLM) that account for population structure and kinship relationships. This approach allowed detection of a significant association between the candidate gene HaRIC_B and SHR incidence (P < 0.01), accounting for a SHR incidence reduction of about 20 %. Conclusions: These results suggest that AM will be useful in dissecting other complex traits in sunflower, thus providing a valuable tool to assist in crop breeding.
Instituto de Biotecnología
Fil: Fusari, Corina Mariana. INTA. Instituto de Biotecnología; Argentina
Fil: Di Rienzo, Julio A. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Troglia, Carolina Beatriz. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Nishinakamasu, Veronica. INTA. Instituto de Biotecnología; Argentina
Fil: Moreno, Maria Valeria. INTA. Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Maringolo, Carla Andrea. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Quiroz, Facundo Jose. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Alvarez, Daniel. INTA. Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Escande, Alberto. INTA. Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Hopp, Horacio Esteban. INTA. Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Heinz, Ruth Amelia. INTA. Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Lia, Veronica Viviana. INTA. Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Paniego, Norma Beatriz. INTA. Instituto de Biotecnología; Argentina
Source
BMC Plant Biology 12 : 93 (2012)
Subject
Helianthus Annuus
Enfermedades de las Plantas
Sclerotinia
Resistencia a la Enfermedad
Loci de Rasgos Cuantitativos
Sclerotinia sclerotiorum
Plant Diseases
Disease Resistance
Quantitative Trait Loci
Girasol
QTL
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/4339