Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2

Pecora, Andrea; Malacari, Darío Amilcar; Pérez Aguirreburualde, María Sol; Bellido, Demian; Nuñez, Maria del Carmen; Dus Santos, Maria Jose; Martínez Escribano, José Angel; Wigdorovitz, Andres
Publication Year
Published version
The aim of this study was to develop and test a multivalent subunit vaccine against Bovine Viral Diarrhea Virus (BVDV) based on the E2 virus glycoprotein belonging to genotypes 1a, 1b and 2a, immunopotentiated by targeting these antigens to antigen-presenting cells. The E2 antigens were expressed in insect cells by a baculovirus vector as fusion proteins with a single chain antibody, named APCH I, which recognizes the β-chain of the MHC Class II antigen. The three chimeric proteins were evaluated for their immunogenicity in a guinea pig model as well as in colostrum-deprived calves. Once the immune response in experimentally vaccinated calves was evaluated, immunized animals were challenged with type 1b or type 2b BVDV in order to study the protection conferred by the experimental vaccine. The recombinant APCH I-tE21a-1b-2a vaccine was immunogenic both in guinea pigs and calves, inducing neutralizing antibodies. After BVDV type 1b and type 2 challenge of vaccinated calves in a proof of concept, the type 1b virus could not be isolated in any animal; meanwhile it was detected in all challenged non-vaccinated control animals. However, the type 2 BVDV was isolated to a lesser extent compared to unvaccinated animals challenged with type 2 BVDV. Clinical signs associated to BVDV, hyperthermia and leukopenia were reduced with respect to controls in all vaccinated calves. Given these results, this multivalent vaccine holds promise for a safe and effective tool to control BVDV in herds
Instituto de Virología
Fil: Pecora, Andrea. INTA. Instituto de Virología; Argentina
Fil: Malacari, Darío Amilcar. INTA. Instituto de Virología; Argentina
Fil: Pérez Aguirreburualde, María Sol. INTA. Instituto de Virología; Argentina
Fil: Bellido, Demian. Vetanco SA; Argentina
Fil: Nuñez, Maria del Carmen. Algenex SL, Madrid; España
Fil: Dus Santos, Maria Jose. INTA. Instituto de Virología; Argentina
Fil: Martínez Escribano, José Angel. Instituto Nacional de Investigación Agraria. Departamento de Biotecnología; España
Fil: Wigdorovitz, Andres. INTA. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Vaccine 33 (39) : 5163-5171. (22 September 2015)
Pestivirus de la Diarrea Bovina
Bovine Diarrhoea Pestivirus
Polyvalent Vaccines
Vacuna Polivalente
E2 Glycoprotein
Access level
Restricted access
INTA Digital (INTA)
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier