Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization

Authors
Nava, Santiago; Beati, Lorenza; Venzal, José Manuel; Labruna, Marcelo B.; Szabó, Matías P.J.; Petney, Trevor; Saracho Bottero, Maria Noelia; Tarragona, Evelina Luisa; Dantas-Torres, Filipe; Silva, Maria M. Santos; Mangold, Atilio Jose; Guglielmone, Alberto; Estrada-Peña, Agustín
Publication Year
2018
Language
English
Format
article
Status
Versión aceptada para publicación
Description
The aims of this work were to re-describe all parasitic stages of Rhipicephalus sanguineus sensu stricto, to select and deposit a neotype, and to characterize some of its diagnostic molecular traits. A male of R. sanguineus s.s. collected in Montpellier, France, was designated as neotype. The diagnostic characters unique to the male of R. sanguineus s.s. are: spiracular plate elongated and subtriangular in shape with a dorsal prolongation narrow and usually visible dorsally, with the dorsal prolongation narrower than the width of the adjacent festoon; punctations of the scutum moderate in number and unequal in size; marginal groove conspicuous, deep and punctate; posteromedian groove distinct and elongated, and posterolateral grooves often sub-circular, shorter than posteromedian groove; adanal plates long, wide, and subtriangular in shape, with a clear concavity in its inner margin and posterior margin broadly rounded or truncated; accessory adanal plates with the posterior end pointed, narrower than the width of adjacent festoon. The female of R. sanguineus s.s. can be diagnosed by a combination of broadly U-shaped genital aperture, spiracular plate with a narrow dorsal prolongation visible dorsally, basis capituli hexagonal with broad lateral angles, and scutum barely longer than broad with posterior margin sinuous and punctations moderate in number and unequal in size, larger and more numerous along cervical fields. The nymph has a basis capituli sub-triangular dorsally with lateral angles slightly curved and presence of ventral processes, scutum approximately as long as broad with lateral margins nearly straights, posterior margin broadly rounded, and cervical grooves short and sigmoid in shape extending posteriorly to the level of the eyes. The larva is characterized by basis capituli broader than long with lateral angles short and slightly curved and with posterior margin slightly convex, cervical grooves short, shallow and subparallel, and scutum almost twice broader than long. The phylogenetic analysis of DNA sequences support R. sanguineus s.s. as a well-defined taxon when compared with other species of the R. sanguineus group: R. turanicus s.s., R. camicasi, R. guilhoni, R. sulcatus, R. pusillus, R. rossicus and R. leporis. Molecularly R. sanguineus s.s. also encompasses the so-called “temperate lineage” from the New World (Argentina, southern Brazil, Chile, Uruguay, and USA). The evidence currently available supports the presence of R. sanguineus s.s. in Europe (France, Italy, Spain, Switzerland and Portugal) and America (Argentina, Brazil, Chile, Uruguay and U.S.A.), but further studies are needed to determine the exact geographic range of this taxon.
EEA Rafaela
Fil: Nava, Santiago. INTA. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beati, Lorenza. Georgia Southern University. Institute for Coastal Plain Science. United States National Tick Collection; Estados Unidos
Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fil: Labruna, Marcelo B. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fil: Szabó, Matías P.J. Universidade Federal de Uberlandia. Faculdade de Medicina Veterinaria; Brasil
Fil: Petney, Trevor. Karlsruhe Institute of Technology. Department of Ecology and Parasitology; Alemania
Fil: Saracho Bottero, Maria Noelia. INTA. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tarragona, Evelina Luisa. INTA. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dantas-Torres, Filipe. Oswaldo Cruz Foundation (Fiocruz). Aggeu Magalhães Institute. Department of Immunology; Brasil
Fil: Silva, Maria M. Santos. Instituto Nacional de Saúde Dr. Ricardo Jorge. Centro de Estudos de Vectores e Doenças Infecciosas Dr. Francisco Cambournac; Portugal
Fil: Mangold, Atilio Jose. INTA. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guglielmone, Alberto. INTA. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Estrada-Peña, Agustin. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España
Source
Ticks and Tick-borne Diseases 9 (6) : 1573-1585 (September 2018)
Subject
Rhipicephalus Sanguineus
Metastigmata
Identificación
Anatomía Animal
Secuencia Nucleotídica
Identification
Animal Morphology
Nucleotide Sequence
Garrapatas
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/3381