Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds

Authors
Cazon, Luis Ignacio; Conforto, Erica Cinthia; Fernandez, Franco Daniel; Paredes, Juan Andrés; Rago, Alejandro Mario
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Thecaphora frezii is the causal agent of peanut smut, important disease in the Argentine production area. The pathogen can be dispersed by kernels, through surface contamination or small lesions. The aim of this work was to design specific primers to develop a Polymerase Chain Reaction assay for the detection of Thecaphora frezii in peanuts kernels. The ITS region of four T. frezii isolates was amplified and sequenced. A consensus sequence was obtained and a pair of primers specific for the pathogen, named TF-2F and TF- 2R, was synthesized. The primers were used for specificity and sensitivity tests. The former test involved a PCR using DNA from T. frezii and other seed-transmitted pathogens (S. rolfsi, S. minor, Fusarium solani and S. sclerotiorum). A 190 bp fragment was obtained for the T. frezii isolates, whereas no amplification was observed for the remaining pathogens. For the sensitivity test, positive results were observed at concentrations up to 3x10-4 pg of the target DNA. The specificity of this method, along with its sensitivity, makes it an important tool for epidemiological studies of peanut smut.
Inst. Patología Vegetal
Fil: Cazon, Luis Ignacio. INTA. Instituto de Patología Vegetal; Argentina
Fil: Conforto, Erica Cinthia. INTA. Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. INTA. Instituto de Patología Vegetal; Argentina
Fil: Paredes, Juan Andrés. INTA. Instituto de Patología Vegetal; Argentina
Fil: Rago, Alejandro Mario INTA. Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Source
Journal of Plant Pathology 98 (2) : 327-330 (2016)
Subject
Enfermedades de las Plantas
Arachis Hypogaea
Genética
Plant Diseases
Genetics
Maní
Thecaphora Frezzi
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/1242