A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA- 2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ pro...

Authors
Gimenez, Alba Marina; Françoso, Katia S.; Ersching, Jonatan; Icimoto, Marcelo Y.; Oliveira, Vitor; Rodriguez, Anabel Elisa; Schnittger, Leonhard; Florin-Christensen, Mónica; Rodrigues, Mauricio M.; Soares, Irene S.
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Background: Babesia bovis is a tick-transmitted protozoan hemoparasite and the causative agent of bovine babesiosis, a potential risk to more than 500 million cattle worldwide. The vaccines currently available are based on attenuated parasites, which are difficult to produce, and are only recommended for use in bovines under one year of age. When used in older animals, these vaccines may cause life-threatening clinical symptoms and eventually death. The development of a multi-subunit recombinant vaccine against B. bovis would be attractive from an economic standpoint and, most importantly, could be recommended for animals of any age. In the present study, recombinant ectodomains of MSA-2a1, MSA-2b and MSA-2c antigens were expressed in Pichia pastoris yeast as secreted soluble peptides. Results: The antigens were purified to homogeneity, and biochemically and immunologically characterized. A vaccine formulation was obtained by emulsifying a mixture of the three peptides with the adjuvant Montanide ISA 720, which elicited high IgG antibody titers against each of the above antigens. IgG antibodies generated against each MSA-antigen recognized merozoites and significantly inhibited the invasion of bovine erythrocytes. Cellular immune responses were also detected, which were characterized by splenic and lymph node CD4+ T cells producing IFN-γ and TNF-α upon stimulation with the antigens MSA-2a1 or MSA-2c.
Inst. de Patobiología
Fil: Gimenez, Alba Marina. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; Brasil
Fil: Françoso, Katia S. Universidade de Sao Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; Brasil
Fil: Ersching, Jonatan. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; Brasil
Fil: Icimoto, Marcelo Y. Universidade Federal de Sao Paulo. Departamento de Biofísica; Brasil
Fil: Oliveira, Vitor. Universidade Federal de Sao Paulo. Departamento de Biofísica; Brasil
Fil: Rodriguez, Anabel Elisa. INTA. Instituto de Patobiología; Argentina
Fil: Schnittger, Leonhard. INTA. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Mónica. INTA. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Françoso, Katia S. Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; Brasil
Fil: Rodrigues, Mauricio M. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; Brasil
Fil: Soares, Irene S. Universidade de Sao Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; Brasil
Source
Parasites and vectors 9 (1) : 577. (2016)
Subject
Babesia Bovis
Enfermedades de los Animales
Vacuna
Antígenos
Anticuerpos
Vaccines
Antigens
Antibodies
Animal Diseases
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/1000