Multiple-Herbicide resistance in a 2,4-D–Resistant waterhemp (Amaranthus tuberculatus) population from Nebraska

Authors
Crespo, Roberto J.; Wingeyer, Ana Beatriz; Kruger, Greg R.; Riggins, Chance W.; Tranel, Patrick J.; Bernards, Mark L.
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose–response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase–inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non–target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors.
EEA Paraná
Fil: Crespo, Roberto J. University of Nebraska–Lincoln. Department of Agronomy and Horticulture; Estados Unidos
Fil: Wingeyer, Ana Beatriz. INTA. Estación Experimental Agropecuaria Paraná; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kruger, Greg R. University of Nebraska–Lincoln. West Central Research and Extension Center; Estados Unidos
Fil: Riggins, Chance W. University of Illinois. Department of Crop Sciences; Estados Unidos
Fil: Tranel, Patrick J. University of Illinois. Department of Crop Sciences; Estados Unidos
Fil: Bernards, Mark L. University of Nebraska–Lincoln. Department of Agronomy and Horticulture; Estados Unidos
Source
Weed Science 65 (6) :743–754. (November 2017)
Subject
Resistencia a los Herbicidas
Resistencia Cruzada
Atrazina
Mesotriona
Glufosinato
Glifosato
Resistance to Herbicides
Cross Resistance
Atrazine
Mesotrione
Glufosinate
Glyphosate
Amaranthus Tuberculatus
Nebraska, Estados Unidos
Cáñamo de Agua
2,4-D
Waterhemp
Imazethapyr
Lactofen
Aminocyclopyrachlor
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/5698