Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest

Authors
Romano, Nelson Gaston; Gioffre, Andrea; Sede, Silvana Mabel; Campos, Eleonora; Cataldi, Angel Adrian; Talia, Paola Mónica
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups including Achromobacter and Pseudomonas genera. All three consortia presented cellulolytic activity. The carboxymethylcellulase (CMCase) and total cellulase activities were studied both quantitatively and qualitatively and optimal enzymatic conditions were characterized and compared among the three consortia. Thermal and pH stability were analyzed. Based on its cellulolytic activity, one consortium was selected for further characterization by zymography. We detected a specific protein of 55 kDa with CMCase activity. In this study, we have shown that these consortia encode for cellulolytic enzymes. These enzymes could be useful for lignocellulosic biomass degradation into simple components and for different industrial applications.
Instituto de Biotecnología
Fil: Romano, Nelson Gaston. INTA. Instituto de Biotecnología; Argentina.
Fil: Gioffre, Andrea Karina. INTA. Instituto de Biotecnología; Argentina
Fil: Sede, Silvana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina
Fil: Campos, Eleonora. INTA. Instituto de Biotecnología; Argentina
Fil: Cataldi, Angel Adrian. INTA. Instituto de Biotecnología; Argentina
Fil: Talia, Paola Mónica. INTA. Instituto de Biotecnología; Argentina
Source
Current Microbiology 67 (2) : 138–147 (August 2013)
Subject
Bosques
Bosque Primario
Pseudomonas
Celulasa
Actividad Enzimática
Forests
Primary Forests
Cellulase
Enzyme Activity
Bosques Nativos
Argentina
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/4272