Diversity, genetic mapping, and signatures of domestication in the carrot (Daucus carota L.) genome, as revealed by Diversity Arrays Technology (DArT) markers

Authors
Grzebelus, Dariusz; Iorizzo, Massimo; Senalik, Douglas A.; Ellison, Shelby L.; Cavagnaro, Pablo; Macko-Podgorni, Alicja; Heller-Uszynska, Kasia; Kilian, Andrzej; Nothnagel, Thomas; Simon, Philipp W.; Baranski, Rafal; Allender, Charlotte
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Carrot is one of the most economically important vegetables worldwide, but genetic and genomic resources supporting carrot breeding remain limited. We developed a Diversity Arrays Technology (DArT) platform for wild and cultivated carrot and used it to investigate genetic diversity and to develop a saturated genetic linkage map of carrot. We analyzed a set of 900 DArT markers in a collection of plant materials comprising 94 cultivated and 65 wild carrot accessions. The accessions were attributed to three separate groups: wild, Eastern cultivated and Western cultivated. Twenty-seven markers showing signatures for selection were identified. They showed a directional shift in frequency from the wild to the cultivated, likely reflecting diversifying selection imposed in the course of domestication. A genetic linkage map constructed using 188 F2 plants comprised 431 markers with an average distance of 1.1 cM, divided into nine linkage groups. Using previously anchored single nucleotide polymorphisms, the linkage groups were physically attributed to the nine carrot chromosomes. A cluster of markers mapping to chromosome 8 showed significant segregation distortion. Two of the 27 DArT markers with signatures for selection were segregating in the mapping population and were localized on chromosomes 2 and 6. Chromosome 2 was previously shown to carry the Vrn1 gene governing the biennial growth habit essential for cultivated carrot. The results reported here provide background for further research on the history of carrot domestication and identify genomic regions potentially important for modern carrot breeding.
EEA La Consulta
Fil: Grzebelus, Dariusz. University of Agriculture in Krakow. Institute of Plant Biology and Biotechnology; Polonia
Fil: Iorizzo, Massimo. University of Wisconsin. Department of Horticulture; Estados Unidos
Fil: Senalik, Douglas. University of Wisconsin. Department of Horticulture; Estados Unidos. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos
Fil: Ellison, Shelby. University of Wisconsin. Department of Horticulture; Estados Unidos
Fil: Cavagnaro, Pablo Federico. . INTA. Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Macko-Podgórni, Alicja. University of Agriculture in Krakow. Institute of Plant Biology and Biotechnology; Polonia
Fil: Heller-Uszynska, Kasia. Diversity Arrays Technology Pty Ltd; Australia
Fil: Kilian, Andrzej. Diversity Arrays Technology Pty Ltd; Australia
Fil: Nothnagel, Thomas. Federal Research Centre for Cultivated Plants. Julius Kühn-Institut. Institute for Breeding Research on Horticultural and Fruit Crops; Alemania
Fil: Allender, Charlotte. University of Warwick. Warwick Crop Centre; Gran Bretaña
Fil: Simon, Philipp. University of Wisconsin. Department of Horticulture; Estados Unidos. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos
Fil: Baranski, Rafal. University of Agriculture in Krakow. Institute of Plant Biology and Biotechnology; Polonia
Source
Molecular Breeding 33 (3) : 625–637 (March 2014)
Subject
Zanahoria
Daucus Carota
Genomas
Marcadores Genéticos
Mapas Genéticos
Variación Genética
Carrots
Genomes
Genetic Markers
Genetic Maps
Genetic Variation
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/3212