Broad resistance to acetohydroxyacid-synthase-inhibiting herbicides in feral radish (Raphanus sativus L.) populations from Argentina

Authors
Pandolfo, Claudio Ezequiel; Presotto, Alejandro; Moreno, Florencia; Dossou, Ida; Migasso, Juan P.; Sakima, Ernesto; Cantamutto, Miguel Angel
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
BACKGROUND: Soon after the commercial release of sunflower cultivars resistant to imidazolinone herbicides, several uncontrolled feral radish (Raphanus sativus L.) populations were found in south-eastern Buenos Aires, Argentina. These populations were studied in field, glasshouse and laboratory experiments aiming to characterise their resistance profile and to develop management tools. RESULTS: Three feral radish accessions were highly resistant to ten active ingredients of five families of acetohydroxyacid synthase (AHAS)-inhibiting herbicides. Sequence analysis of the AHAS gene detected a Trp574Leu mutation in all resistant accessions. One accession with an intermediate level of resistance was heterozygous for this mutation, probably owing to gene exchange with a susceptible subpopulation located in the field margin. Herbicide-resistant and herbicide-susceptible radish could be controlled in sunflower by alternative herbicides. CONCLUSION: This is the first report of feral radish with resistance to herbicides belonging to all the AHAS-inhibiting herbicide families, conferred by Trp574Leu mutation in the AHAS gene. An appropriate herbicide rotation with alternative herbicides such as fluorochloridone or aclonifen and an increase in the diversity of cropping systems are important for minimising the prevalence of these biotypes.
Fil: Pandolfo, Claudio Ezequiel. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigación Científica y Técnica; Argentina
Fil: Presotto, Alejandro. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigación Científica y Técnica; Argentina
Fil: Moreno, Florencia. BASF Argentina S.A.; Argentina.
Fil: Dossou, Ida. DNA LandMarks Inc.; Canada
Fil: Migasso, Juan P. BASF Argentina S.A.; Argentina
Fil: Sakima, Ernesto. BASF Argentina S.A.; Argentina
Fil: Cantamutto, Miguel Angel. INTA. Estación Experimental Agropecuaria Hilario Ascasubi; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigación Científica y Técnica; Argentina
Source
Pest management science 72 (2) : 354-361. (2016 Feb)
Subject
Raphanus Sativus
Resistencia a los Herbicidas
Metsulfuron
Resistance to Herbicides
Argentina
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/2344