Molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentina

Authors
Fernandez, Franco Daniel; Conci, Vilma Cecilia; Kirschbaum, Daniel Santiago; Conci, Luis Rogelio
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Strawberry (Fragaria x ananassa Duch. cv. Camino Real) plants showing phyllody symptoms were detected in production fields in Bella Vista (Corrientes province, Argentina). DNA from all symptomatic samples analyzed yielded fragments of the expected size in PCR reactions using phytoplasma universal primers. DNA from asymptomatic plants yielded no products. The associated phytoplasma was designated as Argentinean Strawberry Phyllody (ASP). The ASP sequence of the 16S rRNA gene showed 99–98% homology with members of the 16SrVII ash yellows group. The putative RFLP profile was indistinguishable from the Argentinean alfalfa witches’ broom (ArAWB) phytoplasma. The phylogenetic analysis of nearly full-length 16S rDNA sequence and 16S-23S spacer region yielded a consensus tree wherein ASP clustered into the ash yellows group (16Sr VII) with high confidence values (95), generating a separated branch (100 bootstrap value) together with ArAWB (16Sr VII-C). In Argentina, the 16Sr VII group was also detected in alfalfa (Medicago sativa L.) crops, and weeds [Artemisa annua L. and Conyza bonariensis (L.) Cronquist]. To our knowledge, this is the first report of a phytoplasma from the 16Sr VII group affecting strawberries in Argentina and worldwide
Fil: Fernandez, Franco Daniel. INTA. Instituto de Patología Vegetal; Argentina
Fil: Conci, Vilma Cecilia. INTA. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kirschbaum, Daniel Santiago. INTA. Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Conci, Luis Rogelio. INTA. Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba; Argentina
Source
European journal of plant pathology 135 (1) : 1–4. (January 2013)
Subject
Fresa
Fitoplasmas
Biología Molecular
Strawberries
Phytoplasmas
Molecular Biology
Frutilla
Argentina
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/1727