Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production

Authors
Grassi, Emanuel Marcelo; Pildain, Maria Belen; Levin, Laura Noemi; Carmaran, Cecilia Cristina
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
In this study the extracellular production of ligninolytic enzymes by xylariaceous and diatrypaceous species from Argentina was investigated. Among them, a new species (Eutypa microasca) was evaluated. Its morphological characteristics (e.g.: very small asci), along with the molecular analyses, support the hypothesis of the new taxon. Ligninolytic enzyme production by several saprophytic and endophytic strains belonging to the genera Xylaria, Eutypella, Eutypa and Peroneutypa was assessed on solid medium supplemented with different dyes (Poly R-478, Azure B and Malachite Green) and under submerged fermentation in copper amended malt extract/glucose medium. All the strains produced laccase in liquid cultures, and seven out of nine also produced Mn-peroxidase (highest titres detected 113 U/l). Only 5 of the strains assayed could not decolorize Malachite Green. This is the first report on laccase and Mn-peroxidase production by the members of the Diatrypaceae and contributes to the knowledge on ligninolytic enzyme production in the members of the Xylariaceae, scarcely studied up to date.
Fil: Grassi, Emanuel Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Fil: Pildain, Maria Belen. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
Fil: Levin, Laura Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Fil: Carmaran, Cecilia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Subject
Diatrypaceae
Eutypa microasca
ligninolytic enzymes
Micología
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/17811