A comparative analysis of sperm selection procedures prior to cryopreservation on Nili-Ravi buffalo bull (Bubalus bubalis) semen. Assessment of its impact on post-thaw sperm functi...

Authors
Husna, Asma U. I.; Ejaz, Rabea; Qadeer, Saima; Azam, Asima; Rakha, Bushra; Ansari, Muhammad Sajjad; Shahzad, Quaisar; Javed, Moazzam; Vazquez, Monica Hebe; Akhter, Shamin
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Sperm selection techniques have been developed to get sperm suspensions enriched in motile and functional cells. Studies show that selection before cryopreservation improves post-thaw quality of cryopreserved sperm but information on buffalo bull sperm is scarce. The study was aimed to 1) perform a comparative analysis of sperm selection procedures; Swim-Up (SU), Sephadex?-G15 Filtration (S-G15) or Glass Wool Filtration (GWF) for total and motile cell recovery, 2) to assess the impact of sperm selection prior to cryopreservation on sperm quality (motility, morphology, cell membrane and normal apical ridge, viability and livability, chromatin integrity) and sperm functionality (Embryo Cleavage after IVF with selected sperm) in post-thawed suspensions of buffalo bull sperm. Semen was collected from 5 Nili Ravi buffalo bulls maintained at the Semen Production Unit Qadirabad, District Sahiwal, Pakistan. Ejaculates were divided into four aliquots for SU, S-G15 and GWF and control. After sperm selection, total and motile sperm recovery was highest in GWF samples (total sperm=84.08±8.39%; motile sperm=80.42±3.57%). An improvement (P<0.05) in all post-thaw parameters was observed in S-G15-selected sperm and, in some parameters in GWF-filtered sperm suspensions compared to control. The highest (P<0.05) embryo cleavage rate (%) was achieved with frozen-thawed sperm selected with S-G15 prior to cryopreservation (44.72±4.18) compared to control (21.98±3.00). In conclusion, post thaw sperm quality was improved after sperm selection from fresh buffalo bull semen through S-G15 and GWF procedures compared to SU and control while, the fertility rate (cleavage rate) was improved with sperm processed using the S-G15 procedure.
Fil: Husna, Asma U. I.. Pir Mehr Ali Shah Arid Agriculture University; Pakistán
Fil: Ejaz, Rabea. Pir Mehr Ali Shah Arid Agriculture University; Pakistán
Fil: Qadeer, Saima. Pir Mehr Ali Shah Arid Agriculture University; Pakistán
Fil: Azam, Asima. Pir Mehr Ali Shah Arid Agriculture University; Pakistán
Fil: Rakha, Bushra. Pir Ali Shah Arid Agriculture University; Pakistán
Fil: Ansari, Muhammad Sajjad. Pir Ali Shah Arid Agriculture University; Pakistán
Fil: Shahzad, Quaisar. Buffalo Research Institute; Pakistán
Fil: Javed, Moazzam. Semen Production Unit; Pakistán
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Akhter, Shamin. Pir Mehr Ali Shah Arid Agriculture University; Pakistán
Subject
BUFFALO BULL
SPERMATOZOA
CRYOPRESERVATION
GLASS WOOL
SELECTION
Bioquímica y Biología Molecular
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Biología Reproductiva
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/24225