Characterization of lactobacilli strains derived from cocoa fermentation in the south of Bahia for the development of probiotic cultures

Authors
Teles Santos, Tiza; Santos Ornelas, Roberta María; Borges Arcucio, Leonardo; Messias Oliveira, Mayara; Nicoli, Jaques Robert; Villela Dias, Cristiano; Trovatti Uetanabaro, Ana Paula; Vinderola, Celso Gabriel
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
In the last years, several factors have contributed to the development of probiotic cultures from locally sourced strains. In this paper, we aimed to characterize Lactobacillus plantarum and Lactobacillus fermentum isolates derived from Brazilian cocoa fermentation for the development of new probiotic cultures. Isolates diversity was studied by RAPD and strains were further tested in vitro for their probiotic potential. Physiological traits such as heat tolerance, hydrophobicity, resistance to simulated gastrointestinal digestion and antibiotic susceptibility were studied. Besides, activity against food pathogens was tested through four different assays: deferred inhibition, co-aggregation, co-cultivation and antagonism of supernatants. Considering the resistance to simulated gastrointestinal digestion and the results from the antimicrobial and co-aggregation tests, L. plantarum 286 showed the most promising results, followed by L. plantarum 289, for further studies for their application as probiotics.
Fil: Teles Santos, Tiza. Universidade Estadual de Santa Cruz; Brasil
Fil: Santos Ornelas, Roberta María. Universidade Estadual de Santa Cruz; Brasil
Fil: Borges Arcucio, Leonardo. Universidade Federal de Minas Gerais; Brasil
Fil: Messias Oliveira, Mayara. Universidade Estadual de Santa Cruz; Brasil
Fil: Nicoli, Jaques Robert. Universidade Federal de Minas Gerais; Brasil
Fil: Villela Dias, Cristiano. Universidade Estadual de Santa Cruz; Brasil
Fil: Trovatti Uetanabaro, Ana Paula. Universidade Estadual de Santa Cruz; Brasil
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Subject
Probiotics
Chocolate
Isolation
Alimentos y Bebidas
Otras Ingenierías y Tecnologías
INGENIERÍAS Y TECNOLOGÍAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/59370