Enhanced Antitumor Activity against Melanoma Cancer Cells by Nitric Oxide Release and Photosensitized Generation of Singlet Oxygen from Ruthenium Complexes

• Authors: Ramos, Loyanne C. B.; Marchesi, Mario Sérgio Pereira; Callejon, Daniel; Baruffi, Marcelo Dias; Lunardi, Claure N.; Slep, Leonardo Daniel; Bendhack, Lusiane M.; da Silva, Roberto Santana
• Publication Year: 2016
• Language: English
• Format: article
• Status: Published version
• Description: We have investigated the synergistic effect of nitric oxide and singlet oxygen originating from stimulated ruthenium complexes and their photodynamic action against a melanoma cancer cell line. In aqueous solution, the photosensitizer [Ru(NH3)5pz]2+(I; pz = pyrazine) and the nitrosylruthenium complex trans-[RuCl([15]aneN4)NO]2+(II; [15]aneN4= 1,4,8,12-tetrazacyclopentadecane) can produce singlet oxygen and nitric oxide, respectively. The phototoxicity of complex I does not correlate with the singlet oxygen quantum yields unless it is mediated by nitric oxide release from complex II. This effect is around 70 and 50 % higher than the effect prompted by compounds I and II, respectively, in isolation in the presence of light. It was found that the number of hypodiploid cells in the melanoma cancer cells increased, which indicates that a mixture of compounds I and II induces apoptosis of tumorigenic cell lines. Both singlet oxygen and nitric oxide produced by the ruthenium complexes enhance the photodynamic efficacy in melanoma cancer cells.; Fil: Ramos, Loyanne C. B.. Universidade de Sao Paulo; Brasil; Fil: Marchesi, Mario Sérgio Pereira. Universidade de Sao Paulo; Brasil; Fil: Callejon, Daniel. Universidade de Sao Paulo; Brasil; Fil: Baruffi, Marcelo Dias. Universidade de Sao Paulo; Brasil; Fil: Lunardi, Claure N.. Universidade do Brasília; Brasil; Fil: Slep, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina; Fil: Bendhack, Lusiane M.. Universidade de Sao Paulo; Brasil; Fil: da Silva, Roberto Santana. Universidade de Sao Paulo; Brasil
• Subject: ANTITUMOR AGENTS; NITROGEN OXIDES; RUTHENIUM; SINGLET OXYGEN; TOXICITY; Otras Ciencias Químicas; Ciencias Químicas; CIENCIAS NATURALES Y EXACTAS
• Access level: Restricted access
• License: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
• Institution: Consejo Nacional de Investigaciones Científicas y Técnicas
• OAI Identifier: oai:ri.conicet.gov.ar:11336/59084