Enhanced Antitumor Activity against Melanoma Cancer Cells by Nitric Oxide Release and Photosensitized Generation of Singlet Oxygen from Ruthenium Complexes

Authors
Ramos, Loyanne C. B.; Marchesi, Mario Sérgio Pereira; Callejon, Daniel; Baruffi, Marcelo Dias; Lunardi, Claure N.; Slep, Leonardo Daniel; Bendhack, Lusiane M.; da Silva, Roberto Santana
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
We have investigated the synergistic effect of nitric oxide and singlet oxygen originating from stimulated ruthenium complexes and their photodynamic action against a melanoma cancer cell line. In aqueous solution, the photosensitizer [Ru(NH3)5pz]2+(I; pz = pyrazine) and the nitrosylruthenium complex trans-[RuCl([15]aneN4)NO]2+(II; [15]aneN4= 1,4,8,12-tetrazacyclopentadecane) can produce singlet oxygen and nitric oxide, respectively. The phototoxicity of complex I does not correlate with the singlet oxygen quantum yields unless it is mediated by nitric oxide release from complex II. This effect is around 70 and 50 % higher than the effect prompted by compounds I and II, respectively, in isolation in the presence of light. It was found that the number of hypodiploid cells in the melanoma cancer cells increased, which indicates that a mixture of compounds I and II induces apoptosis of tumorigenic cell lines. Both singlet oxygen and nitric oxide produced by the ruthenium complexes enhance the photodynamic efficacy in melanoma cancer cells.
Fil: Ramos, Loyanne C. B.. Universidade de Sao Paulo; Brasil
Fil: Marchesi, Mario Sérgio Pereira. Universidade de Sao Paulo; Brasil
Fil: Callejon, Daniel. Universidade de Sao Paulo; Brasil
Fil: Baruffi, Marcelo Dias. Universidade de Sao Paulo; Brasil
Fil: Lunardi, Claure N.. Universidade do Brasília; Brasil
Fil: Slep, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina
Fil: Bendhack, Lusiane M.. Universidade de Sao Paulo; Brasil
Fil: da Silva, Roberto Santana. Universidade de Sao Paulo; Brasil
Subject
ANTITUMOR AGENTS
NITROGEN OXIDES
RUTHENIUM
SINGLET OXYGEN
TOXICITY
Otras Ciencias Químicas
Ciencias Químicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/59084