Nitrosylation: An adverse factor in Uremic Haemolytic Syndrome. Antitoxin effect of Ziziphus mistol Griseb

Autores
Aiassa, Virginia; Albrecht, Claudia; Bustos, Pamela Soledad; Ortega, María Gabriela; Eraso, Alberto Jorge; Albesa, Ines
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
Versión publicada
Descripción
Toxins of Escherichia coli (STEC) causing Uremic Hemolytic Syndrome (UHS) generate oxidative stress in human blood with more production of nitric oxide (NO) than reactive oxygen species (ROS). Shiga toxin (Stx) together with the hemolysin (Hly) increased lipid oxidation, as evaluated by malondialdehyde MDA and oxidation of proteins. The addition of Ziziphus mistol Griseb extracts decreased NO, ROS, MDA and simultaneously caused an increase in the degradation of oxidized proteins to advanced oxidation protein products (AOPPs) in controls and samples with toxins. Furthermore, the nitrosylated proteins/AOPP ratio was reduced, due to the increase of AOPP. Z. mistol Griseb extracts exhibited a high proportion of polyphenols and flavonoids, with evident correlation with ferrous reduction antioxidant potential (FRAP). The plasma of eight children with UHS showed oxidative stress and NO stimulus, comparable to the effect of toxins during the assays in vitro. UHS children presented high levels of nitrosylated proteins respect to control children of similar age. Although the degradation of oxidized proteins to AOPP rose in UHS children,the nitrosylated proteins/AOPP rate increased as a consequence of the elevated nitrosative stress observed in these patients.
Fil: Aiassa, Virginia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina
Fil: Albrecht, Claudia. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Escuela de Nutricion; Argentina
Fil: Bustos, Pamela Soledad. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina
Fil: Ortega, María Gabriela. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina
Fil: Eraso, Alberto Jorge. Universidad Nacional de Cordoba. Facultad de Ciencias Medicas. Escuela de Nutricion; Argentina
Fil: Albesa, Ines. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina
Materia
UREMIC HEMOLYTIC SYNDROME
ESCHERICHIA COLI
ZIZIPHUS MISTOL GRISEB
NITROSYLATION
OXIDATIVE STRESS
ADVANCED OXIDATION PROTEIN PRODUCTS
Nutrición, Dietética
Ciencias de la Salud
CIENCIAS MÉDICAS Y DE LA SALUD
Nivel de accesibilidad
Acceso restringido
Licencia
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11161