Biocontrol of fungi isolated from sour rot infected table grapes by Saccharomyces and other yeast species

Authors
Nally, Maria Cristina; Pesce, Virginia Mercedes; Maturano, Yolanda Paola; Toro, Maria Eugenia; Combina, Mariana; Castellanos de Figueroa, Lucia Ines; Vazquez, Fabio
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Sour rot is an important disease of grapes caused by an etiologic complex of microorganisms in which filamentous fungi play a key role. Yeasts are used for biocontrol of pathogenic filamentous fungi on fruits. The major objective of this study was to assess in vivo on detached berries the effect of viticultural yeasts on phytopathogenic fungi involved in grape sour rot. Yeasts that were found to be effective in vivo againstthe fungi were assayed for their possible pathogenicity in humans: growth at 42 ◦C, pseudohyphal formation, adhesion, and phospholipase and protease activity.Atotal of 234 yeasts belonging to 14 genera were assayed against the following pathogens: Aspergillus caelatus, Aspergillus carbonarius, Aspergillus terreus, Aspergillus versicolor, Fusarium oxysporum, Penicillium comune, Rhizopus stolonifer and Ulocladium sp. Forty-three (16 Saccharomyces and 27 non-Saccharomyces) showed antagonistic properties against some of the fungi assayed in grapes at 25 ◦C. Yeast isolates determined as biocontrol agents under in vivo conditions were isolated from fermenting musts (35), viticultural soils (6) and grape berries (2). Twenty biocontrol agents did not show phenotypical characteristics associated with pathogenicity in humans.
Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina;
Fil: Pesce, Virginia Mercedes. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina;
Fil: Maturano, Yolanda Paola. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza. Centro de Estudios Enológicos; Argentina; Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina;
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina;
Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza. Centro de Estudios Enológicos; Argentina;
Fil: Castellanos de Figueroa, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina; Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina;
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina;
Subject
Biocontrol
Fungi
Table grapes
Saccharomyces
Micología
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Horticultura, Viticultura
Agricultura, Silvicultura y Pesca
CIENCIAS AGRÍCOLAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/1805