Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells

Authors
Villena, Julio Cesar; Suzuki, Rie; Fujie, Hitomi; Chiba, Eriko; Takahashi, Takuya; Tomosada, Yohsuke; Shimazu, Tomoyuki; Aso, Hisashi; Ohwada, Shuichi; Suda, Yoshihito; Ikegami, Shuji; Itoh, Hiroyuki; Alvarez, Gladis Susana; Saito, Tadao; Kitazawa, Haruki
Publication Year
2012
Language
English
Format
article
Status
Published version
Description
Previously, we demonstrated that Lactobacillus (L.) jensenii TL2937 attenuates the inflammatory response triggered by activation of toll-like receptor (TLR)-4 in porcine intestinal epithelial cells. In view of the critical importance of antigen-presenting cells (APCs) polarization in immunoregulation, the objective of the present study was to examine the effect of TL2937 strain on activation patterns of APCs from swine Peyer's patches (PPs). We demonstrated that direct exposure of porcine APCs to L. jensenii in the absence of inflammatory signals increased expression of IL-10 and TGF-β in CD172a+ APCs and caused them to display tolerogenic properties. In addition, pre-treatment of CD172a+ APCs with L. jensenii resulted in differential modulation of the production of pro- and anti-inflammatory cytokines in response to TLR4 activation. The immunomodulatory effect of TL2937 strain was not related to a downregulation of TLR4 but an upregulation of the expression of three negative regulators of TLRs: SIGIRR, A20, and IRAK-M. Our results also indicated that TLR2 has an important role in the anti-inflammatory activity of L. jensenii TL2937 since anti-TLR2 antibodies blocked the upregulation of SIGIRR and IRAK-M in CD172a+ APCs and the production of IL-10 in response to TLR4 activation. We performed, for the first time, a precise functional characterization of porcine APCs from PPs, and we demonstrated that CD172a+ cells were tolerogenic. Our findings demonstrate that adherent cells and isolated CD172a+ cells harvested from swine PPs were useful for in vitro study of the inflammatory responses in the porcine gut and the immunomodulatory effects of immunobiotic microorganisms.
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Suzuki, Rie. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Fujie, Hitomi. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Chiba, Eriko. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Takahashi, Takuya. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Tomosada, Yohsuke. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Shimazu, Tomoyuki. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Ohwada, Shuichi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environmental Science; Japón
Fil: Ikegami, Shuji. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Itoh, Hiroyuki. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Saito, Tadao. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Kitazawa, Haruki. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Subject
LACTOBACILLUS JENSENII
PORCINE ANTIGEN PRESENTING CELLS
ANTI-INFLAMMATORY ACTIVITY
TOLL-LIKE RECEPTOR 4
Bioquímica y Biología Molecular
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/24931