ALS herbicide resistance mutations in Raphanus raphanistrum: Evaluation of pleiotropic effects on vegetative growth and ALS activity

Authors
Li, Mei; Yu, Qin; Han, Heping; Vila Aiub, Martin Miguel; Powles, Stephen B.
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Background Gene mutations that endow herbicide resistance may cause pleiotropic effects on plant ecology and physiology. This paper reports on the effect of a number of known and novel target-site resistance mutations of the ALS gene (Ala-122-Tyr, Pro-197-Ser, Asp-376-Glu or Trp-574-Leu) on vegetative growth traits of the weed Raphanus raphanistrum. Results The results from a series of experiments have indicated that none of these ALS resistance mutations imposes negative pleiotropic effects on relative growth rate (RGR), photosynthesis and resource-competitive ability in R. raphanistrum plants. The absence of pleiotropic effects on plant growth occurs in spite of increased (Ala-122-Tyr, Pro-197-Ser, Asp-376-Glu) and decreased (Trp-574-Leu) extractable ALS activity. Conclusion The absence of detrimental pleiotropic effects on plant growth associated with the ALS target-site resistance mutations reported here is a contributing factor in resistance alleles being at relatively high frequencies in ALS-herbicide-unselected R. raphanistrum populations.
Fil: Li, Mei. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia. Shandong Academy of Agricultural Sciences. Institute of Plant Protection; China
Fil: Yu, Qin. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Han, Heping. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Vila Aiub, Martin Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Powles, Stephen B.. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Subject
Acetolactate synthase (ALS)
Adaptation
ALS activity
Mutations
Resistance allele
Resistance mechanism
Target-site mutation
Ecología
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/4259