Methylation status regulates lipoprotein lipase expression in chronic lymphocytic leukemia

Authors
Abreu, Cecilia; Moreno, Pilar; Palacios, Florencia; Borge, Mercedes; Morande, Pablo Elías; Landoni, Ana Inés; Gabus, Raul; Dighiero, Guillermo; Giordano, Mirta Nilda; Gamberale, Romina; Oppezzo, Pablo
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Among different prognostic factors in chronic lymphocytic leukemia (CLL), we previously demonstrated that lipoprotein lipase (LPL) is associated with an unmutated immunoglobulin profile and clinical poor outcome. Despite the usefulness of LPL for CLL prognosis, its functional role and the molecular mechanism regulating its expression are still open questions. Interaction of CLL B-cells with the tissue microenvironment favors disease progression by promoting malignant B-cell growth. Since tissue methylation can be altered by environmental factors, we investigated the methylation status of the LPL gene and the possibility that overexpression could be associated with microenvironment signals. Our results show that a demethylated state of the LPL gene is responsible for its anomalous expression in unmutated CLL cases and that this expression is dependent on microenvironment signals. Overall, this work proposes that an epigenetic mechanism, triggered by the microenvironment, regulates LPL expression in CLL disease.
Fil: Abreu, Cecilia. Instituto Pasteur de Montevideo; Uruguay
Fil: Moreno, Pilar. Instituto Pasteur de Montevideo; Uruguay
Fil: Palacios, Florencia. Instituto Pasteur de Montevideo; Uruguay
Fil: Borge, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Morande, Pablo Elías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Landoni, Ana Inés. Hospital Maciel; Uruguay
Fil: Gabus, Raul. Hospital Maciel; Uruguay
Fil: Dighiero, Guillermo. Hospital Maciel; Uruguay
Fil: Giordano, Mirta Nilda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Gamberale, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Oppezzo, Pablo. Universidad de la República; Uruguay
Subject
LIPOPROTEIN LIPASE
LEUKEMIA
METHYLATION
MICROENVIRONMENT
PROGNOSTIC FACTORS
Patología
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/20863