Latex Particles Sensitized with Proteins of Leptospira interrogans for Application in Immunoagglutination Assays

Authors
Prochetto, Santiago; Gonzalez, Veronica Doris Guadalupe; Garcia, Valeria Soledad; Vanasco, Norma Bibiana; Lottersberger, Javier; Gugliotta, Luis Marcelino
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
Physical adsorption and chemical coupling of recombinant proteins of Leptospira interrogans onto polystyrene and core-shell carboxylated particles were, respectively, investigated with the aim of producing latex?protein complexes to be used in immunoagglutination assays able to detect the leptospirosis disease in either humans or animals. To this effect, a protein lysate of crude extracts was evaluated, and sensitizations were carried out at different pHs, with the antigenic proteins approach to the particle surface favored at pH close to their isoelectric point. In the covalent coupling experiments, high fractions of proteins were chemically bound to carboxyl groups on the particle surface and higher densities of linked proteins were obtained for particles exhibiting greater carboxyl group densities. The produced latex?protein complexes were tested in immunoagglutination assays, by turbidimetry and a visual method, from a panel that included positive and negative bovine, canine, and human sera. The area under receiver operating characteristic curves was used as an index of accuracy. The complexes obtained by covalent coupling of proteins on the latex of higher density of carboxyl groups allowed an acceptable discrimination between the studied positive and negative sera.
Fil: Prochetto, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Gonzalez, Veronica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Garcia, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Vanasco, Norma Bibiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Lottersberger, Javier. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Subject
Immunoagglutination
latex-protein complex
sensitized particles
Leptospirosis
Otras Ingeniería de los Materiales
Ingeniería de los Materiales
INGENIERÍAS Y TECNOLOGÍAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/20488