A simple Streptomyces spore-based impedimetric biosensor to detect indane pesticide

Authors
López Rodriguez, Maria Lorena; Benimeli, Claudia Susana; Madrid, Rossana Elena; Giacomelli, Carla Eugenia
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
Lindane is a pesticide potentially harmful to environment and human health and in general, its detection involves mostly tedious, expensive and time-consuming techniques. A new strategy to detect lindane can be focused on the activity of Streptomyces strain M7 (SM7), which is capable to grow in the presence of organochlorine pesticides (OCPs) as carbon source. On this regard, the aim of this work is to present an optimized design of a microbial biosensor prototype based on a SM7 spore platform to detect and quantify lindane by electrochemical impedance spectroscopy (EIS) as the transduction method. By means of this technique, the metabolic bacterial activity was evaluated by analyzing a set of electrical parameters calculated by fitting the measured impedance spectra through an equivalent electrical circuit. Activity studies were performed by incubating the SM7 spore platform with (optimized incubation conditions) and without (to be used in a practical biosensor) shaking in the presence of lindane. In all cases, bacterial activity was measured by the impedance changes produced in the cells due to lindane removal from the culture medium. This proposed label-free impedimetric biosensor is simple, cost-effective and feasible to detect changes in the lindane concentration as low as 120 μg L−1 in less than 2 days without sample pretreatment. In addition, the surface of electrodes can be reused for several different tests. This sensor prototype represents a valuable alternative for OCPs detection.
Fil: López Rodriguez, Maria Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Benimeli, Claudia Susana. Universidad del Norte Santo Tomás de Aquino; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Madrid, Rossana Elena. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnologia. Departamento de Bioingenieria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Giacomelli, Carla Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; Argentina
Subject
Streptomyces
Impedimetric biosensor
Spore-based platform
Lindane pesticide
Impedance spectroscopy
Electrochemical detection
Físico-Química, Ciencia de los Polímeros, Electroquímica
Ciencias Químicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/12468