Continuous flow generation of magnetoliposomes in a low-cost portable microfluidic platform

Authors
Conde, Alvaro José; Battalla, Milena; Cerda, Maria Belen; Mykhaylyk, Olga; Plank, Christian; Podhajcer, Osvaldo Luis; Cabaleiro, Juan Martin; Madrid, Rossana Elena; Policastro, Lucia Laura
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
We present a low-cost, portable microfluidic platform that uses laminated polymethylmethacrylate chips, peristaltic micropumps and LEGO® Mindstorms components for the generation of magnetoliposomes that does not require extrusion steps. Mixtures of lipids reconstituted in ethanol and an aqueous phase were injected independently in order to generate a combination of laminar flows in such a way that we could effectively achieve four hydrodynamic focused nanovesicle generation streams. Monodisperse magnetoliposomes with characteristics comparable to those obtained by traditional methods have been obtained. The magnetoliposomes are responsive to external magnetic field gradients, a result that suggests that the nanovesicles can be used in research and applications in nanomedicine.
Fil: Conde, Alvaro José. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnologia. Departamento de Bioingenieria. Laboratorio de Medios E Interfases; Argentina
Fil: Battalla, Milena. Comision Nacional de Energia Atomica. Gerencia Quimica. CAC; Argentina
Fil: Cerda, Maria Belen. Comision Nacional de Energia Atomica. Gerencia Quimica. CAC; Argentina
Fil: Mykhaylyk, Olga. Technische Universität München. Institute of Experimental Oncology and Therapy Research; Alemania
Fil: Plank, Christian. Technische Universität München. Institute of Experimental Oncology and Therapy Research; Alemania
Fil: Podhajcer, Osvaldo Luis. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Cabaleiro, Juan Martin. Universidad de Buenos Aires. Facultad de Ingenieria. Departamento de Ingenieria Mecanica. Laboratorio de Fluidodinamica; Argentina
Fil: Madrid, Rossana Elena. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnologia. Departamento de Bioingenieria. Laboratorio de Medios E Interfases; Argentina
Fil: Policastro, Lucia Laura. Comision Nacional de Energia Atomica. Gerencia Quimica. CAC; Argentina
Subject
MICROFLUIDICS
MAGNETOLIPOSOMES
NANOVESICLES
METHACRYLATE
Tecnología de Laboratorios Médicos
Ingeniería Médica
INGENIERÍAS Y TECNOLOGÍAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/8733