Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range

Autores
Casero, Carina Noelia; Estévez-Braun, Ana; Gutiérrez Ravelo, Ángel; Demo, Mirta; Méndez Alvarez, Sabastián; Machin, Félix
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
Versión publicada
Descripción
Currently, there is a pressing need for novel antibacterial agents against drug-resistant bacteria, especially those which have been common in our communities and hospitals, such as methicillin-resistant Staphylococcus aureus (MRSA). The South American plant Achyrocline satureioides (Marcela) has been widely used in traditional medicine for a number of diseases, including infections. Several crude extracts from this plant have shown good antimicrobial activities in vitro. In the search for the active principle (s) that confers these antimicrobial activities, we have processed the dichloromethane extract from the aerial parts of the plant. One of the isolated compounds showed extraordinary antibacterial activities against a set of clinically relevant Gram-positive strains that widely differ in their antibiogram profiles. This compound was identified as achyrofuran on the basis of its spectroscopic and physical data.
We determined the MIC to be around 0.1 M (0.07 g/ml) for the reference methicillin-resistant and
vancomycin-intermediate S. aureus strain NRS402. Moreover, nanomolar concentrations of  achyrofuran killed 10 6 bacteria within 12 h. Based on the presence of the 2,2-biphenol core, we further studied whether achyrofuran killed bacteria through a mechanism of action similar to that reported for the naturally occurring antibiotic MC21-A. Indeed, we found that achyrofuran was not bacteriolytic by itself although it greatly compromised membrane impermeability as determined by increased SYTOX Green uptake.
Fil: Casero, Carina Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Rio Cuarto; Argentina. Universidad de la Laguna; España
Fil: Estévez-Braun, Ana. Universidad de la Laguna; España. Instituto Canario de Investigación del Cáncer; España
Fil: Gutiérrez Ravelo, Ángel. Universidad de la Laguna; España. Instituto Canario de Investigación del Cáncer; España
Fil: Demo, Mirta. Universidad Nacional de Rio Cuarto; Argentina
Fil: Méndez Alvarez, Sabastián. Universidad de la Laguna; España. Hospital Universitario Nuestra Señora de Candelaria; España
Fil: Machin, Félix. Hospital Universitario Nuestra Señora de Candelaria; España
Materia
ACHYROCLINE SATUREIOIDES
STAPHYLOCOCCUS AUREUS
MRSA
PHYTOALEXINS
ACHYROFURAN
ANTIMICROBIAL
Biología Celular, Microbiología
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Nivel de accesibilidad
Acceso restringido
Licencia
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12235