Ochratoxin A production by Aspergillus niger: Effect of water activity and a biopreserver formulated with Lactobacillus plantarum CRL 778

Authors
Gerez, Carla Luciana; Dallagnol, Andrea Micaela; Ponsone, Maria Lorena; Chulze, Sofia Noemi; Font, Graciela Maria
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Some filamentous fungi of the genera Aspergillus (A.) are of high impact in food safety due to the production of a toxic secondary metabolite, the ochratoxin A (OTA). The present study was carried out to evaluate the effect water activity (aw) on the growth and the OTA production by the strain Aspergillus niger 13D both invitro assays and in bread slices. High values of aw favoured growth and OTA production by A.niger 13D in Czapek Yeast extract agar medium (CYA) with the optimum at 0.995. In bread slices, fungi growth rate (μmax: 0.90-1.07cmd-1) and OTA production (5.13-10.79ngg-1) were lower respect to CYA medium (μmax: 1.09-1.81cmd-1, OTA concentration: 26.02-133.28ngg-1) independently to aw. Furthermore, efficacy antifungal of biopreserver formulated with Lactobacillus plantarum CRL 778 (SL778) for packed bread was evaluated. Our result evidenced the inhibitory activity of SL778 against growth of ochratoxigenic A.niger 13D in bread making at different aw. In addition, this biopreserver demonstrated is principally antimycotoxigenic, observing a reduction 60% of OTA at 0.995 and 0.971 aw in bread added with SL778.
Fil: Gerez, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Dallagnol, Andrea Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Ponsone, Maria Lorena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Subject
BREAD
LACTIC ACID BACTERIA
OCHRATOXIN A
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/37928