Immunobiotic bifidobacteria strains modulate rotavirus immune response in porcine intestinal epitheliocytes via pattern recognition receptor signaling

Authors
Ishizuka, Takamasa; Kanmani, Paulraj; Kobayashi, Hisakazu; Miyazaki, Ayako; Soma, Junichi; Suda, Yoshihito; Aso, Hisashi; Nochi, Tomonori; Iwabuchi, Noriyuki; Xiao, Jin-zhong; Saito, Tadao; Villena, Julio Cesar; Haruki Kitazawa
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
In this work, we aimed to characterize the antiviral response of an originally established porcine intestinal epithelial cell line (PIE cells) by evaluating the molecular innate immune response to rotavirus (RVs). In addition, we aimed to select immunomodulatory bacteria with antiviral capabilities. PIE cells were inoculated with RVs isolated from different host species and the infective titers and the molecular innate immune response were evaluated. In addition, the protection against RVs infection and the modulation of immune response by different lactic acid bacteria (LAB) strains was studied. The RVs strains OSU (porcine) and UK (bovine) effectively infected PIE cells. Our results also showed that RVs infection in PIE cells triggered TLR3-, RIG-I- and MDA-5-mediated immune responses with activation of IRF3 and NF-κB, induction of IFN-β and up-regulation of the interferon stimulated genes MxA and RNase L. Among the LAB strains tested, Bifidobacterium infantis MCC12 and B. breve MCC1274 significantly reduced RVs titers in infected PIE cells. The beneficial effects of both bifidobacteria were associated with reduction of A20 expression, and improvements of IRF-3 activation, IFN-β production, and MxA and RNase L expressions. These results indicate the value of PIE cells for studying RVs molecular innate immune response in pigs and for the selection of beneficial bacteria with antiviral capabilities.
Fil: Ishizuka, Takamasa. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Kobayashi, Hisakazu. Tohoku University; Japón
Fil: Miyazaki, Ayako. National Institute of Animal Health. Viral Diseases and Epidemiology Research Division; Japón
Fil: Soma, Junichi. National Federation of Agricultural Cooperative Associations. Institute of Animal Health. Research and Development Section; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Nochi, Tomonori. Tohoku University; Japón
Fil: Iwabuchi, Noriyuki. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
Fil: Xiao, Jin-zhong. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Haruki Kitazawa. Tohoku University; Japón
Subject
IMMUNOBIOTICS
BIFIDOBACTERIA
ROTAVIRUS
PIE CELLS
TLR3
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/78743