Role of Cox-2 in Vascular Inflammation: An Experimental Model of Metabolic Syndrome

Authors
Renna, Nicolas Federico; Diez, Emiliano Raúl; Lembo, Carina; Miatello, Roberto Miguel
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
The objective of this work was to demonstrate the role of COX-2 enzyme at the vascular in experimental model of metabolic syndrome. SHR male WKY rats were employed; they were distributed in 8 groups (N = 8 each): control (W); W + L: WKY rats receiving 20mg/kg of lumiracoxib by intraesophageal administration; SHR; SHR + L: SHR + 20mg/kg of lumiracoxib by intraesophageal administration; Fructose-Fed Rats (FFR):WKYrats receiving 10% (w/v) fructose solution in drinking water during all 12 weeks; FFR + L: FFR + 20mg/kg of lumiracoxib by intraesophageal administration; Fructose-Fed Hypertensive Rats (FFHR): SHR receiving 10% (w/v) fructose solution in drinking water during all 12 weeks; and FFHR + L: FFHR + 20mg/kg of lumiracoxib by intraesophageal administration.Metabolic variables, blood pressure,morphometric variables, and oxidative stress variables were evaluated; alsoMMP-2 andMMP-9 (collagenases), VCAM-1, and NF-B byWesternblot or IFI were evaluated. FFHR presented all variables of metabolic syndrome; there was also an increase in oxidative stress variables; vascular remodeling and left ventricular hypertrophy were evidenced along with a significant increase in the expression of the mentioned proinflammatory molecules and increased activity and expression of collagenase. Lumiracoxib was able to reverse vascular remodeling changes and inflammation, demonstrating the involvement of COX-2 in the pathophysiology of vascular remodeling in this experimental model.
Fil: Renna, Nicolas Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina;
Fil: Diez, Emiliano Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad Nacional de Cuyo; Argentina;
Fil: Lembo, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina;
Fil: Miatello, Roberto Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina; Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina;
Subject
MMP-2 MMP-9
FRUCTOSE-FED HYPERTENSIVE RATS
VASCULAR REMODELING AND INFLAMMATION
COX-2
Patología
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/1867