Improvement of enterocin P purification process

Authors
Cuozzo, Sergio Antonio; Calvez, S.; Prévost, H.; Drider, D.
Publication Year
2006
Language
English
Format
article
Status
Published version
Description
Purification and heterologous expression of enterocin P (EntP), a sec-dependent bacteriocin produced byEnterococcus faecium, inEscherichia coli is described. PCR-amplified product of the enterocin P structural geneentP was cloned into plasmid pET-32b under the control of the inducible T7/ac promoter. The neo-synthesized EntP was genetically modified by an addition of 3 extra amino acids, leading to recombinant EntRP. Active EntRP was recovered from the cytoplasmic soluble fraction ofE. coli harboring appropriate recombinant plasmid, characterized by ELISA and Western-blot analysis and purified by immunoaffinity chromatography. The use ofE. coli as heterologous host and pET-32b as expressing vector offers promising tools for heterologous production of class IIa bacteriocin.
Fil: Cuozzo, Sergio Antonio. Centre National de la Recherche Scientifique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Ecole Nationale des Ingénieurs des Industries des Techniques Agricoles et Alimentaires; Francia
Fil: Calvez, S.. Ecole Nationale des Ingénieurs des Industries des Techniques Agricoles et Alimentaires; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Prévost, H.. Ecole Nationale des Ingénieurs des Industries des Techniques Agricoles et Alimentaires; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Drider, D.. Ecole Nationale des Ingénieurs des Industries des Techniques Agricoles et Alimentaires; Francia. Centre National de la Recherche Scientifique; Francia
Subject
Enterococcus Faecium
Disulfide Bridge
Immobilize Metal Affinity Chromatography
Extra Amino Acid
NcoI Restriction Site
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/43503