Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry

Authors
Porro, Valentina; Pagotto, Romina María del Luján; Harreguy, María Belén; Ramírez, Sofía; Crispo, Martina; Santamaría, Clarisa Guillermina; Luque, Enrique Hugo; Rodriguez, Horacio Adolfo; Bollati Fogolín, Mariela
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
Oct4 is involved in regulation of pluripotency during normal development and is down-regulated during formation of postnatal reservoir of germ cells. We propose thatOct4/GFP transgenic mouse, which mimics the endogenous expression pattern of Oct4, could be used as a mammalian model to study the effects of environmental estrogens on the development of male germ cells. Oct4/GFP maturation profile was assessed during postnatal days -PND- 3, 5, 7, 10, 14 and 80, using flow cytometry. Then, we exposed pregnant mothers to 17α-ethinylestradiol (EE2) from day post coitum (dpc) 5 to PND7. Percentage of Oct4/GFP-expressing cells and levels of expression of Oct4/GPF were increased in PND7 after EE2 exposure. These observations were confirmed by analysis of GFP and endogenous Oct4 protein in the seminiferous tubules and by a reduction in epididymal sperm count in adult mice. We introduced Oct4/GFP mouse together with flow cytometry as a tool to evaluate changes in male germ cells development.
Fil: Porro, Valentina. Instituto Pasteur de Montevideo; Uruguay
Fil: Pagotto, Romina María del Luján. Instituto Pasteur de Montevideo; Uruguay
Fil: Harreguy, María Belén. Instituto Pasteur de Montevideo; Uruguay
Fil: Ramírez, Sofía. Instituto Pasteur de Montevideo; Uruguay
Fil: Crispo, Martina. Instituto Pasteur de Montevideo; Uruguay
Fil: Santamaría, Clarisa Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Rodriguez, Horacio Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Bollati Fogolín, Mariela. Instituto Pasteur de Montevideo; Uruguay
Subject
OCT4
GFP
MALE GERM CELLS
FLOW CYTOMETRY
MATURATION ESTROGENS
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/44506