Functional properties of Lactobacillus plantarum strains: A study in vitro of heat stress influence

Authors
Ferrando, Verónica Andrea; Quiberoni, Andrea del Lujan; Reinheimer, Jorge Alberto; Suárez, Viviana Beatriz
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Functional properties of two Lactobacillus plantarum strains (Lp 813 and Lp 998) were evaluated ( in vitro) before and after heat adaptation and shock. The stress conditions were selected considering a previous work: 55 °C - 15 min (heat shock) and 45 °C - 30 min (thermal adaptation), both performed in MRS broth. The functional properties evaluated were: a-survival to simulated gastrointestinal tract (GIT: saliva + bovine pepsina solution brought to pH 2.3 in 90 min, bile solution 1% w/v pH 8-10 min and bile + pancreatin solution pH 8-30 min), b-co-aggregation with Saccharomyces cerevisiae to simulate intestinal epithelia adhesion, c- auto- and co-aggregation with enteric pathogens ( Escherichia coli and Salmonella enteritidis) and d-use of prebiotic compounds (inulin, soluble corn fiber, lactulose and raffinose) evaluated for kinetics of growth. Heat treatments (generally) affected the studied functional properties as follows: a-diminished the resistance to acidic step and improved survival undergoing bile-pancreatin step in GIT simulation, b-mean values of auto-aggregation and co-aggregation with yeast or pathogens were not significantly modified, despite an increase in variability and c-prebiotic use diminished in the case of strains subjected tothermal treatments except for raffinose, which showed to have been better employed in this last case. These results could be very useful when it comes to the selection of microorganisms to be used and conserved in processes and methodologies that involve high temperatures as a stress factor (spray drying or cheese elaboration).
Fil: Ferrando, Verónica Andrea. Universidad Nacional del Litoral; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Suárez, Viviana Beatriz. Universidad Nacional del Litoral; Argentina
Subject
HEAT CELL ADAPTATION
HEAT STRESS
LACTIC ACID BACTERIA
LACTOBACILLUS PLANTARUM
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/59476