GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration

Authors
Scolz, Massimilano; Widlund, Per O.; Piazza, Silvano; Bublik, Debora Rosa; Reber, Simone; Peche, Leticia Y.; Ciani, Yari; Hubner, Nina; Isokane, Mayumi; Monte, Martin; Ellenberg, Jan; Hyman, Anthony A.; Schneider, Claudio; Bird, Alexander W.
Publication Year
2012
Language
English
Format
article
Status
Published version
Description
The regulation of cell migration is a highly complex process that is often compromised when cancer cells become metastatic. The microtubule cytoskeleton is necessary for cell migration, but how microtubules and microtubule-associated proteins regulate multiple pathways promoting cell migration remains unclear. Microtubule plus-end binding proteins (+TIPs) are emerging as important players in many cellular functions, including cell migration. Here we identify a +TIP, GTSE1, that promotes cell migration. GTSE1 accumulates at growing microtubule plus ends through interaction with the EB1+TIP. The EB1-dependent +TIP activity of GTSE1 is required for cell migration, as well as for microtubule-dependent disassembly of focal adhesions. GTSE1 protein levels determine the migratory capacity of both nontransformed and breast cancer cell lines. In breast cancers, increased GTSE1 expression correlates with invasive potential, tumor stage, and time to distant metastasis, suggesting that misregulation of GTSE1 expression could be associated with increased invasive potential. © 2012 Scolz et al.
Fil: Scolz, Massimilano. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia
Fil: Widlund, Per O.. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Piazza, Silvano. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia
Fil: Bublik, Debora Rosa. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia
Fil: Reber, Simone. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Peche, Leticia Y.. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia
Fil: Ciani, Yari. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia
Fil: Hubner, Nina. Universitair Medisch Centrum Utrecht; Países Bajos
Fil: Isokane, Mayumi. European Molecular Biology Laboratory; Alemania
Fil: Monte, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia
Fil: Ellenberg, Jan. European Molecular Biology Laboratory; Alemania
Fil: Hyman, Anthony A.. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Schneider, Claudio. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia. University of Udine; Italia
Fil: Bird, Alexander W.. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Subject
GTSE
MIGRACION
METASTASIS
MICROTUBULOS
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/66649