Combination of oviduct fluid and heparin to improve monospermic zygotes production during porcine in vitro fertilization

Authors
Tavares Pereira Batista, Ribrio Ivan; Moro, Lucía Natalia; Corbin, Emilie; Alminana, Carmen; Gonçalves Souza Fabjan, Joanna Maria; de Figueirêdo Freitas, Vicente José; Mermillod, Pascal
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
In vivo, the oviduct provides appropriate microenvironment conditions for monospermic fertilization and early embryo development. In addition, glycosaminoglycans such as heparin are present in the oviduct and have been shown to modulate the activity of oviduct-secreted proteins on the regulation of sperms parameters. Thus, the present study was designed to evaluate the effect of porcine oocytes exposure to oviduct fluid (OF) before in vitro fertilization (IVF; incubation of oocytes in OF for 30 minutes before IVF), during IVF (supplementation of IVF medium with 10% OF), and during IVF in combination with heparin (10% OF + 10-μg/mL heparin) on IVF parameters. Regardless of sperm concentration used (0.5, 1.5, or 4.5 × 105 cells/mL), exposure of oocytes to OF led to an increased (P < 0.05) monospermy rate, without alteration (P > 0.05) of the penetration rate in comparison with the control group. This resulted in a general increase (P < 0.05) in the final output of the IVF system in terms of zygotes with two pronuclei in OF-exposed groups: 56 ± 9% (OF before) and 60 ± 7% (10% OF during IVF), compared with control (21 ± 8%), when IVF was performed with 4.5 × 105 cells/mL. The combination of 10% OF with heparin during IVF induced a decrease (P < 0.05) of the penetration rate, with no effect (P > 0.05) on the monospermy rate in comparison with 10% OF alone. This resulted in a general reduction (P < 0.05) in the final output of the IVF system (%), which was 33 ± 6% and 52 ± 8%, for 10% OF + heparin and 10% OF, respectively. In conclusion, the OF, used in porcine IVF, exerted a beneficial effect on oocytes by reducing the incidence of polyspermy without decreasing the penetration rate. However, the association of the OF with heparin reduced the efficiency of monospermic zygotes' production.
Fil: Tavares Pereira Batista, Ribrio Ivan. Centre National de la Recherche Scientifique; Francia. Universidade Estadual Do Ceara; Brasil
Fil: Moro, Lucía Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique; Francia
Fil: Corbin, Emilie. Centre National de la Recherche Scientifique; Francia
Fil: Alminana, Carmen. Centre National de la Recherche Scientifique; Francia
Fil: Gonçalves Souza Fabjan, Joanna Maria. Universidade Estadual Do Ceara; Brasil
Fil: de Figueirêdo Freitas, Vicente José. Universidade Estadual Do Ceara; Brasil
Fil: Mermillod, Pascal. Centre National de la Recherche Scientifique; Francia
Subject
ZONA PELLUCIDA MATURATION
SPERM-OOCYTE INTERACTION
OVIDUCTAL FLUID
PRONUCLEI
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/46635