Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis

Authors
Podio, Maricel; Cáceres, María Emilia; Samoluk, Sergio Sebastián; Seijo, Jose Guillermo; Pessino, Silvina Claudia; Ortiz, Juan Pablo Amelio; Pupilli, Fulvio
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Apomixis, a clonal plant reproduction by seeds, is controlled in Paspalum spp. by a single and recombinally-blocked locus. Partial sequence analysis of the apomixis locus revealed structural features of heterochromatin: i.e. presence of repetitive elements, gene degeneration and de-regulation. To test the epigenetic control of apomixis, a study on the distribution of cytosine methylation at the apomixis locus and the effect of artificial DNA de-methylation on the mode of reproduction was undertaken in two apomictic Paspalum species. The 5-Methyl cytosine distribution in the apomixis-controlling genomic region was studied in P. simplex by methylation-sensitive RFLP analysis and in P. notatum by FISH. The effect of DNA de-methylation was studied on the mode of reproduction of P. simplex by progeny test analysis of apomictic plants treated with the de-methylating agent 5’-Azacytidine. High level of cytosine methylation was detected at the apomixis-controlling genomic region in both species. By analyzing a total of 374 open pollination progenies we found that artificial de-methylation had little or no effect on apospory, whereas it induced a significant depression of parthenogenesis. Our results suggested that factors controlling repression of parthenogenesis might be inactivated in apomictic Paspalum by DNA methylation.
Fil: Podio, Maricel. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Cáceres, María Emilia. CNR-Istituto di Bioscienze e Biorisorse; Italia
Fil: Samoluk, Sergio Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina
Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Pupilli, Fulvio. CNR-Istituto di Bioscienze e Biorisorse; Italia
Subject
APOMIXIS
PASPALUM
EPIGENETIC
5’-AZACYTIDINE
BIII HYBRIDS
DNA METHYLATION
PARTHENOGENESIS
Otras Ciencias Agrícolas
Otras Ciencias Agrícolas
CIENCIAS AGRÍCOLAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/2733