First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis

Authors
Bustos, Carla Paola; Guida, Nora; Casamayor, Almudena; Muñoz, Alejandra Jimena; Fernández Garayzábal, José Francisco; Vela Alonso, Ana Isabel
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
Strangles is one of the most frequently diagnosed equine respiratory infectious diseases in the world. It is caused by Streptococcus equi subsp. equi (S. equi), and it is an acute infection characterized by pyrexia, nasal discharge, pharyngitis, and abscessation of lymph nodes. Frequently, healthy horses might continue to harbor S. equi after clinical recovery. Although the genetic distance between S. equi isolates is short, strains can be differentiated by pulsed-field gel electrophoresis (PFGE) and single locus sequence typing for epidemiological studies. The aim of this study was to characterize by PFGE Argentine isolates of S. equi obtained from horses with acute strangles and those that had recovered. Bacterial isolation and identification of 80 S. equi isolates by phenotypic and genotypic tests were performed using samples from 29 horses with acute strangles and 95 from healthy animals. Also, the isolates were characterized by PFGE using Bsp120I and SmaI. Visual comparison of macrorestriction patterns generated with both enzymes revealed three different DNA fragment profiles with variations of one or two bands. Interestingly, an identical profile was found in isolates from the same horse and from horses that were infected at the same time, and the horses recovered from strangles continue to carry the same strain. Some vaccinated horses have been mild infected for a different strain from that of carriers suggesting other source of infection. This is the first molecular characterization of Argentine isolates of S. equi, which shows the presence of three strains between 2010 and 2013 in Buenos Aires.
Fil: Bustos, Carla Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Guida, Nora. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Casamayor, Almudena. Universidad Complutense de Madrid; España
Fil: Muñoz, Alejandra Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Fernández Garayzábal, José Francisco. Universidad Complutense de Madrid; España
Fil: Vela Alonso, Ana Isabel. Universidad Complutense de Madrid; España
Subject
EPIDEMIOLOGY
PFGE
STRANGLES
STREPTOCOCCUS EQUI SUBSP. EQUI
Ciencias Veterinarias
Ciencias Veterinarias
CIENCIAS AGRÍCOLAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/72825