Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae

Authors
Sequeiros, Cynthia; Garcés, Marisa Elisabeth; Vallejo, Marisol; Marguet, Emilio R.; Olivera, Nelda Lila
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Bacteriocin-producing Lactococcus lactis TW34 was isolated from marine fsh. TW34 bacteriocin inhibited the growth of the fsh pathogen Lactococcus garvieae at 5 AU/ml (minimum inhibitory concentration), whereas the minimum bactericidal concentration was 10 AU/ml. Addition of TW34 bacteriocin to L. garvieae cultures resulted in a decrease of six orders of magnitude of viable cells counts demonstrating a bactericidal mode of action. The direct detection of the bacteriocin activity by Tricine-SDS-PAGE showed an active peptide with a molec- ular mass ca. 4.5 kDa. The analysis by MALDI-TOF-MS detected a strong signal at m/z 2,351.2 that corresponded to the nisin leader peptide mass without the initiating methio- nine, whose sequence STKDFNLDLVSVSKKDSGASPR was confrmed by MS/MS. Sequence analysis of nisin structural gene confrmed that L. lactis TW34 was a nisin Z producer. This nisin Z-producing strain with probiotic properties might be considered as an alternative in the pre- vention of lactococcosis, a global disease in aquaculture systems.
Fil: Sequeiros, Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Garcés, Marisa Elisabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Vallejo, Marisol. Universidad Nacional de la Patagonia San Juan Bosco. Sede Trelew; Argentina
Fil: Marguet, Emilio R.. Universidad Nacional de la Patagonia San Juan Bosco. Sede Trelew; Argentina
Fil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Subject
PROBIOTIC
LACTIC ACID BACTERIA
BACTERIOCIN
LACTOCOCCUS LACTIS
LACTOCOCCUS GARVIEAE
FISH PATHOGEN
Biología Celular, Microbiología
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/10356