Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules

Authors
Zarate, Gabriela del Valle; Nader, Maria Elena Fatima
Publication Year
2006
Language
English
Format
article
Status
Published version
Description
In the present study we investigated the survival and stability of probiotic properties of three vaginal lactobacilli after freeze drying with the addition of individual and combined compounds commonly used as pharmaceutical excipients and/or starters cryoprotectants, and subsequent refrigerated storage during 15 months. Lactobacilli, suspended in lactose, skim milk, ascorbic acid and combinations of them, were freeze-dried, packed into gelatin capsules and stored at 5 °C under darkness. At regular intervals throughout storage, freeze-dried samples were rehydrated and viability, adhesion to vaginal epithelial cells and antimicrobial activities were evaluated. The Lactobacillus tested conserved high viability up to 12 months in capsules containing ascorbic acid or combined excipients whereas lyophilization and storage with lactose or skim milk significantly decreased their survival. Abilities to produce lactic acid, H2O2 and bacteriocin were affected to different extents depending on the condition assayed. Lyophilization and storage also reduced the capacity of lactobacilli to adhere to vaginal epithelial cells but this property was partially restored after the first subculture in broth. The results obtained suggests that suitable selection of excipients that could also act as protecting agents of LAB during storage should be a valuable step in the development of a probiotic formulation with a stable shelf-life.
Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Subject
LACTOBACILLUS
LYOPHILIZATION
PROBIOTIC
STORAGE
SURVIVAL
VAGINA
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/36578