Crx broadly modulates the pineal transcriptome

Authors
Rovsing, Louise; Clokie, Samuel; Bustos, Diego Martin; Rohde, Kristian; Coon, Steven L.; Litman, Thomas; Rath, Martin F.; Møller, Morten; Klein, David C.
Publication Year
2011
Language
English
Format
article
Status
Published version
Description
Cone-rod homeobox (Crx) encodes Crx, a transcription factor expressed selectively in retinal photoreceptors and pinealocytes, the major cell type of the pineal gland. In this study, the influence of Crx on the mammalian pineal gland was studied by light and electron microscopy and by use of microarray and qRTPCR technology, thereby extending previous studies on selected genes (Furukawa 1999). Deletion of Crx was not found to alter pineal morphology, but was found to broadly modulate the mouse pineal transcriptome, characterized by a > 2-fold down-regulation of 543 genes and a > 2-fold up-regulation of 745 genes (p < 0.05). Of these, one of the most highly up-regulated (18-fold) was Hoxc4, a member of the Hox gene family, members of which are known to control gene expression cascades. During a 24-h period, a set of 51 genes exhibited differential day/night expression in pineal glands of wild-type animals; only eight of these were also day/night expressed in the Crx -/- pineal gland. However, in the Crx-/- pineal gland 41 genes exhibited differential night/day expression that was not seen in wild-type animals. These findings indicate that Crx broadly modulates the pineal transcriptome and also influences differential night/day gene expression in this tissue. Some effects of Crx deletion on the pineal transcriptome might be mediated by Hoxc4 up-regulation.
Fil: Rovsing, Louise. Universidad de Copenhagen; Dinamarca. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Clokie, Samuel. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Bustos, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Rohde, Kristian. Universidad de Copenhagen; Dinamarca
Fil: Coon, Steven L.. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Litman, Thomas. Universidad de Copenhagen; Dinamarca
Fil: Rath, Martin F.. Universidad de Copenhagen; Dinamarca
Fil: Møller, Morten. Universidad de Copenhagen; Dinamarca
Fil: Klein, David C.. National Instituto of Child Health & Human Development; Estados Unidos
Subject
CRX
GENE EXPRESSION
HOXC4
MICROARRAY
PINEAL GLAND
TRANSCRIPTOME PROFILING
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/81345