Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin

Authors
Rodríguez, Ernesto; Carasi, Paula; Frigerio, Sofía; da Costa, Valeria; van Vliet, Sandra; Noya, Verónica; Brossard, Natalie; van Kooyk, Yvette; García Vallejo, Juan J.; Freire, Teresa
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that F. hepatica glycans increase Th2 immune responses by immunomodulating TLR-induced maturation and function of dendritic cells (DCs). This process was mediated by the macrophage Gal/GalNAc lectin (MGL) expressed on DCs, which recognizes the Tn antigen (GalNAc-Ser/Thr) on parasite components. More interestingly, we identified MGL-expressing CD11c+ cells in infected animals and showed that these cells are recruited both to the peritoneum and the liver upon F. hepatica infection. These cells express the regulatory cytokines IL-10, TNFα and TGFβ and a variety of regulatory markers. Furthermore, MGL+ CD11c+ cells expand parasite-specific Th2/regulatory cells and suppress Th1 polarization. The results presented here suggest a potential role of MGL in the immunomodulation of DCs induced by F. hepatica and contribute to a better understanding of the molecular and immunoregulatory mechanisms induced by this parasite.
Fil: Rodríguez, Ernesto. Universidad de la República; Uruguay
Fil: Carasi, Paula. Universidad de la República; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Frigerio, Sofía. Universidad de la República; Uruguay
Fil: da Costa, Valeria. Universidad de la República; Uruguay
Fil: van Vliet, Sandra. VU University Medical Center; Países Bajos
Fil: Noya, Verónica. Universidad de la República; Uruguay
Fil: Brossard, Natalie. Universidad de la República; Uruguay
Fil: van Kooyk, Yvette. VU University Medical Center; Países Bajos
Fil: García Vallejo, Juan J.. VU University Medical Center; Países Bajos
Fil: Freire, Teresa. Universidad de la República; Uruguay
Subject
C-TYPE LECTIN RECEPTORS
DENDRITIC CELL
GLYCANS
HELMINTH
IMMUNE REGULATION
MACROPHAGE GAL/GALNAC LECTIN
Inmunología
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Otras Ciencias de la Salud
Ciencias de la Salud
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/56479