A communal catalogue reveals Earth's multiscale microbial diversity

Authors
Thompson, Luke R.; Sanders, Jon G.; McDonald, Daniel; Amir, Amnon; Ladau, Joshua; Locey, Kenneth J.; Prill, Robert J.; Tripathi, Anupriya; Gibbons, Sean M.; Ackermann, Gail; Navas Molina, Jose A.; Janssen, Stefan; Kopylova, Evguenia; Vázquez Baeza, Yoshiki; González, Antonio; Morton, James T.; Mirarab, Siavash; Xu, Zhenjiang Zech; Jiang, Lingjing; Haroon, Mohamed F.; Kanbar, Jad; Zhu, Qiyun; Song, Se Jin; Kosciolek, Tomasz; Bokulich, Nicholas A.; Lefler, Joshua; Brislawn, Colin J.; Humphrey, Gregory; Owens, Sarah M.; Hampton Marcell, Jarrad; Berg Lyons, Donna; McKenzie, Valerie; Fierer, Noah; Fuhrman, Jed A.; Clauset, Aaron; Stevens, Rick L.; Shade, Ashley; Pollard, Katherine S.; Goodwin, Kelly D.; Jansson, Janet K.; Gilbert, Jack A.; Knight, Rob; Dionisi, Hebe Monica; Lozada, Mariana; Mac Cormack, Walter Patricio
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
Our growing awareness of the microbial world's importance and diversity contrasts starkly with our limited understanding of its fundamental structure. Despite recent advances in DNA sequencing, a lack of standardized protocols and common analytical frameworks impedes comparisons among studies, hindering the development of global inferences about microbial life on Earth. Here we present a meta-analysis of microbial community samples collected by hundreds of researchers for the Earth Microbiome Project. Coordinated protocols and new analytical methods, particularly the use of exact sequences instead of clustered operational taxonomic units, enable bacterial and archaeal ribosomal RNA gene sequences to be followed across multiple studies and allow us to explore patterns of diversity at an unprecedented scale. The result is both a reference database giving global context to DNA sequence data and a framework for incorporating data from future studies, fostering increasingly complete characterization of Earth's microbial diversity.
Fil: Thompson, Luke R.. University of California at San Diego; Estados Unidos. University of Southern Mississippi; Estados Unidos. National Oceanic and Atmospheric Administration; Estados Unidos
Fil: Sanders, Jon G.. University of California at San Diego; Estados Unidos
Fil: McDonald, Daniel. University of California at San Diego; Estados Unidos
Fil: Amir, Amnon. University of California at San Diego; Estados Unidos
Fil: Ladau, Joshua. University of California San Francisco; Estados Unidos
Fil: Locey, Kenneth J.. Indiana University; Estados Unidos
Fil: Prill, Robert J.. IBM Almaden Research Center; Estados Unidos
Fil: Tripathi, Anupriya. University of California at San Diego; Estados Unidos
Fil: Gibbons, Sean M.. Massachusetts Institute of Technology; Estados Unidos
Fil: Ackermann, Gail. University of California San Diego; Estados Unidos
Fil: Navas Molina, Jose A.. University of California at San Diego; Estados Unidos
Fil: Janssen, Stefan. University of California at San Diego; Estados Unidos
Fil: Kopylova, Evguenia. University of California at San Diego; Estados Unidos
Fil: Vázquez Baeza, Yoshiki. University of California at San Diego; Estados Unidos
Fil: González, Antonio. University of California at San Diego; Estados Unidos
Fil: Morton, James T.. University of California at San Diego; Estados Unidos
Fil: Mirarab, Siavash. University of California at San Diego; Estados Unidos
Fil: Xu, Zhenjiang Zech. University of California at San Diego; Estados Unidos
Fil: Jiang, Lingjing. University of California at San Diego; Estados Unidos
Fil: Haroon, Mohamed F.. Harvard University; Estados Unidos
Fil: Kanbar, Jad. University of California at San Diego; Estados Unidos
Fil: Zhu, Qiyun. University of California at San Diego; Estados Unidos
Fil: Song, Se Jin. University of California at San Diego; Estados Unidos
Fil: Kosciolek, Tomasz. University of California at San Diego; Estados Unidos
Fil: Bokulich, Nicholas A.. Northern Arizona University; Estados Unidos
Fil: Lefler, Joshua. University of California at San Diego; Estados Unidos
Fil: Brislawn, Colin J.. Earth and Biological Sciences Directorate; Estados Unidos
Fil: Humphrey, Gregory. University of California at San Diego; Estados Unidos
Fil: Owens, Sarah M.. Argonne National Laboratory; Estados Unidos
Fil: Hampton Marcell, Jarrad. Argonne National Laboratory; Estados Unidos. University of Illinois at Chicago; Estados Unidos
Fil: Berg Lyons, Donna. University of Colorado; Estados Unidos
Fil: McKenzie, Valerie. University of Colorado; Estados Unidos
Fil: Fierer, Noah. University of Colorado; Estados Unidos
Fil: Fuhrman, Jed A.. University of Colorado; Estados Unidos
Fil: Clauset, Aaron. University of Colorado; Estados Unidos
Fil: Stevens, Rick L.. Argonne National Laboratory; Estados Unidos. University of Chicago; Estados Unidos
Fil: Shade, Ashley. Michigan State University; Estados Unidos
Fil: Pollard, Katherine S.. The Gladstone Institutes; Estados Unidos. University of California San Francisco; Estados Unidos
Fil: Goodwin, Kelly D.. National Oceanic and Atmospheric Administration; Estados Unidos
Fil: Jansson, Janet K.. Earth and Biological Sciences Directorate; Estados Unidos
Fil: Gilbert, Jack A.. Argonne National Laboratory; Estados Unidos. University of Chicago; Estados Unidos
Fil: Knight, Rob. University of California San Diego; Estados Unidos
Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Mac Cormack, Walter Patricio. Universidad de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Subject
MICROBIAL DIVERSITY
EARTH MICROBIOME PROJECT
AMPLICON SEQUENCING
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/65846