A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel

Authors
Aguirre, Andres; Cabruja, Matias Ezequiel; Cabrera, Rodolfo; Eberhardt, Maria Florencia; Peirú, Salvador; Menzella, Hugo Gabriel; Rasia, Rodolfo Maximiliano
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Steryl glucosides (SG) are common contaminants in biodiesel that form precipitates, which form and cause problems due to fouling during transport and storage. Therefore, their quantification is necessary to assess the quality of this fuel. The methods currently available for SG analysis require expensive instrumentation, need a previous concentration step by solid-phase extraction (SPE) or are of limited use for the quantitative assessment. We developed an enzymatic method for SG quantification in biodiesel samples based on the hydrolysis of the glucoside catalyzed by a broadly specific beta glucosidase and the subsequent determination of the glucose released by the reaction. The method is non-expensive, sensitive and was adapted to 96-well format fluorescence plate reader, making it useful for the parallel assay of multiple samples. The enzymatic assay presented here represent a valuable tool for both quality control and the development of improved biodiesel production and purification procedures.
Fil: Aguirre, Andres. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; Argentina
Fil: Cabruja, Matias Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Cabrera, Rodolfo. Unitec Bio S.A; Argentina
Fil: Eberhardt, Maria Florencia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; Argentina
Fil: Peirú, Salvador. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; Argentina
Fil: Menzella, Hugo Gabriel. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A; Argentina
Fil: Rasia, Rodolfo Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Subject
BIODIESEL
STERYL GLUCOSIDES
FLUORESCENCE
NADPH
Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
Biotecnología Industrial
INGENIERÍAS Y TECNOLOGÍAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/8625